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Perturbations of peripheral B lymphocyte homoeostasis in children with systemic lupus erythematosus
  1. M Odendahl1,
  2. R Keitzer2,
  3. U Wahn2,
  4. F Hiepe3,
  5. A Radbruch1,
  6. T Dörner3,*,
  7. R Bunikowski2,*
  1. 1Deutsches Rheuma-Forschungszentrum Berlin, Germany
  2. 2Department of Paediatric Pneumology and Immunology, Charité, Campus Virchow-Klinikum, Humboldt University of Berlin, Germany
  3. 3Department of Rheumatology and Clinical Immunology, Charité University Hospital Berlin, Germany
  1. Correspondence to:
    Dr T Dörner, Department of Medicine, Rheumatology and Clinical Immunology, Charité University Hospital Berlin, Schumannstr 20/21, 10098 Berlin, Germany;
    thomas.doerner{at}charite.de

Abstract

Objective: To investigate the distribution of peripheral B cell subpopulations of children with active and inactive systemic lupus erythematosus (SLE) compared with healthy controls.

Methods: Peripheral B cell subpopulations of 11 children with SLE (6 with active and 5 with inactive disease) and 14 age matched normal healthy children were analysed. Active disease was diagnosed in children with a flare of SLE, who received treatment by IV cyclophosphamide or IV methylprednisolone pulse to control the disease. Additionally, the peripheral B cells of the children with SLE were compared with those of 13 consecutive patients with adult onset SLE.

Results: No major difference was found in the frequency and total number of CD27/CD19+ naïve B cells and CD27+/CD19+ memory B cells between patients with active and inactive lupus and healthy controls, but there was a significant increase in CD27high expressing plasma blasts in patients with active SLE. These cells coexpress CD38+, HLA-DRdim, surface Iglow and lack the expression of CD20 but are clearly positive for intracellular Ig, indicative of early plasma cells. Most CD138+ cells coexpress CD27high/CD19+. The enhanced frequency of peripheral plasma blasts in children with active SLE is consistent with previous findings in adult patients with SLE, whereas a relative predominance of CD27+ memory B cells was only identified in the adult patients.

Conclusions: Profound abnormalities in the distribution of B cell compartments are more pronounced in older patients with SLE, but an enhanced frequency and cell number of peripheral plasma blasts is characteristic of both diseases during active stages. Thus detection of CD27high plasma blasts significantly correlates with active lupus in both children and adults.

  • systemic lupus erythematosus
  • lymphocytes
  • B cells
  • immunological memory
  • CD27
  • BSA, bovine serum albumin
  • Ig, immunoglobulin
  • NHS, normal healthy subjects
  • PBMC, peripheral blood mononuclear cells
  • PBS, phosphate buffered saline
  • PE, phycoerythrin
  • SLE, systemic lupus erythematosus

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Footnotes

  • * T Dörner and R Bunikowski contributed equally to this work