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Modulation of cell recruitment by anti-inflammatory agents in antigen-induced arthritis
  1. M J López-Armada,
  2. O Sánchez-Pernaute,
  3. R Largo,
  4. I Diez-Ortego,
  5. I Palacios,
  6. J Egido,
  7. G Herrero-Beaumont
  1. Inflammation Research Unit, Fundación Jiménez Díaz, Universidad Autónoma, Madrid, Spain
  1. Correspondence to:
    Dr G Herrero-Beaumont, Fundación Jiménez Díaz, Reyes Católicos, 2 28040 Madrid, Spain;
    gherrero{at}fjd.es

Abstract

Objective: To study the effect of meloxicam (MXC) and diclofenac (DCF) on the recruitment of leucocytes during acute experimental arthritis.

Methods: Rabbits with antigen-induced arthritis were treated with MXC, DCF, or not treated. After 48 hours, synovial fluid (SF) leucocyte influx and prostaglandin E2 (PGE2) levels were evaluated. Interleukin 8 (IL8) and monocyte chemotactic peptide-1 (MCP-1) expression and synthesis were studied in the inflamed tissues.

Results: Arthritic knees showed synovial effusion with a high leucocyte count and PGE2 concentration, and an increased expression of IL8 and MCP-1. Both non-steroidal anti-inflammatory drugs (NSAIDs) reduced PGE2 levels and the polymorphonuclear cell (PMN) concentration in SF, while the mononuclear cell (MN) concentration was unchanged in the treated groups in comparison with controls. A definite reduction of IL8 levels was obtained with the treatments, but the drugs did not prevent the up regulation of MCP-1.

Conclusion: The effect of these NSAIDs in acute arthritis may be related to the down regulation of IL8 production. The results suggest a differential effect of anti-inflammatory drugs on PMN and MN recruitment to the joint.

  • chemokines
  • cyclo-oxygenase
  • prostaglandin E2
  • arthritis
  • COX, cyclo-oxygenase
  • DCF, diclofenac
  • IL, interleukin
  • MCP-1, monocyte chemotactic peptide-1
  • MN, mononuclear cells
  • MXC, meloxicam
  • NSAIDs, non-steroidal anti-inflammatory drugs
  • PGE2
  • prostaglandin E2
  • PMN, polymorphonuclear cells
  • SF, synovial fluid
  • SM, synovial membrane

https://doi.org/10.1136/ard.61.11.1027

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