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Macrophage inflammatory protein 1 alpha expression by synovial fluid neutrophils in rheumatoid arthritis
  1. Yoshimi Hatanoa,
  2. Tsuyoshi Kasamaa,
  3. Hideaki Iwabuchia,
  4. Ryosuke Hanaokaa,
  5. Hiroko T Takeuchia,
  6. Lu Jinga,
  7. Yoshiaki Morib,
  8. Kazuo Kobayashic,
  9. Masao Negishia,
  10. Hirotsugu Idea,
  11. Mitsuru Adachia
  1. aThe First Department of Internal Medicine, Showa University School of Medicine, Tokyo, Japan, bDepartment of Physical Medicine and Rehabilitation, Showa University School of Medicine, Tokyo, Japan, cDepartment of Host Defenses, National Institute of Infectious Diseases, Tokyo, Japan
  1. Dr T Kasama, The First Department of Internal Medicine Showa University School of Medicine, 1–5–8 Hatanodai, Shinagawa-ku, Tokyo 142–8666, Japan.

Abstract

OBJECTIVE To determine the contribution made by synovial fluid (SF) neutrophils to the augmented expression of macrophage inflammatory protein 1 α (MIP-1α) in rheumatoid arthritis (RA).

METHODS Neutrophils were isolated from samples of SF from RA patients and peripheral blood (PB) samples from RA patients and healthy controls. Cell associated MIP-1α was visualised immunohistochemically, and cell associated MIP-1α as well as MIP-1α secreted into the SF was assayed by ELISA. Steady state expression of MIP-1α mRNA was assessed by reverse transcription polymerase chain reaction (RT-PCR).

RESULTS Freshly isolated SF neutrophils contained significantly higher concentrations of both MIP-1α protein and its transcript than PB neutrophils from either RA patients or healthy controls; incubation in the absence or presence of tumour necrosis factor α for 24 hours resulted in a significant increase in MIP-1α secretion by RA SF neutrophils compared with neutrophils obtained from either normal PB or RA PB; and expression of MIP-1α by SF neutrophils was well correlated with both RA disease activity and SF mononuclear cell (MNC) counts.

CONCLUSION Expression and secretion of MIP-1α by SF neutrophils may be indicative of local and systemic inflammation in RA. Moreover, this C-C chemokine may contribute to the recruitment of MNCs from the bloodstream into synovial joints and tissues.

  • neutrophil, MIP-1α
  • rheumatoid arthritis
  • chemokine

https://doi.org/10.1136/ard.58.5.297

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