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  • Examination of synovial fluid and serum hyaluronidase activity as a joint marker in rheumatoid arthritis and osteoarthritis patients (by zymography)

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Examination of synovial fluid and serum hyaluronidase activity as a joint marker in rheumatoid arthritis and osteoarthritis patients (by zymography)
  1. Hiroshige Nagayaa,
  2. Tatsuya Ymagatab,
  3. Sadako Ymagatab,
  4. Kazuto Iyodac,
  5. Haruo Itoc,
  6. Yukiharu Hasegawaa,
  7. Hisashi Iwataa
  1. aDepartment of Orthopaedic Surgery, Nagoya University school of Medicine, Nagoya, Aichi, Japan, bDepartment of Biomolecular Engineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan, cDepartment of Orthopaedic Surgery, The Tokyo Kosei Nenkin Hospital, Tokyo, Japan
  1. Dr H Nagaya, Department of Orthopaedic Surgery, Nagoya University School of Medicine, 65 Tsurumai-cho, syouwa-ku, Nagoya, Aichi 466–8550, Japan.

Abstract

OBJECTIVE Hyaluronic acid (HA) is an important joint marker and the substrate for hyaluronidase (HAase). Synovial fluid (SF) and serum HAase were measured to investigate the potential use of HAase as a joint marker in rheumatoid arthritis (RA) and osteoarthritis (OA) patients.

METHODS The subjects were 39 patients with RA and 42 patients with OA. HAase activity was measured by zymography and its relation with various parameters examined statistically.

RESULTS In RA SF a positive correlation (r=0.458, p=0.0186) was found between SF HAase activity and the concentration of serum C reactive protein. A positive correlation (r=0.45, p=0.024) was also found between SF HAase activity and platelet count in the RA group. Serum HAase activity in the RA group was significantly higher than in the OA group (p<0.0001) and normal controls (p<0.0001).

CONCLUSION The results suggest that SF HAase activity could be used as a marker of synovial inflammation.

  • hyaluronidase
  • zymography
  • rheumatoid arthritis

https://doi.org/10.1136/ard.58.3.186

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