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  • Reduced purine 5’-nucleotidase activity in lymphocytes of patients with systemic lupus erythematosus: results of a pilot study

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Reduced purine 5’-nucleotidase activity in lymphocytes of patients with systemic lupus erythematosus: results of a pilot study
  1. Jan N Stolka,
  2. Diana G M de Koningb,
  3. Arie H Penningsc,
  4. Ronney A De Abreub,
  5. Leo B A van de Puttea,
  6. Agnes M Th Boerboomsa
  1. aDepartments of Rheumatology, bPaediatrics (Laboratory of Purine and Pyrimidine Metabolism), cand Haematology, dUniversity Hospital Nijmegen, Nijmegen, the Netherlands
  1. Dr J N Stolk, Department of Rheumatology, University Hospital Nijmegen, PO Box 9101, 6500 HB Nijmegen, the Netherlands.

Abstract

OBJECTIVE To investigate purine metabolism in patients with systemic lupus erythematosus (SLE) for possible abnormalities that might be related to their overall impaired immune function.

METHODS This pilot study included 17 patients with SLE (2 men, 15 women). Enzyme activities of the purine enzymes 5’-nucleotidase (5’NT), purine nucleoside phosphorylase (PNP), and hypoxanthine-guanine-phosphoribosyltransferase (HGPRT) were measured in peripheral blood mononuclear cells (PBMC) and also in fractions of T cells (differentiation antigen CD3+) (n = 12) and B cells (CD19+) (n = 9). The activity of the thiopurine enzyme thiopurine-methyltransferase (TPMT) was measured in red cell lysate. Routine blood tests and indices of disease activity were measured as well. Results were compared with those of healthy volunteers.

RESULTS Compared with their controls, the female SLE patients had a more than 50% reduced activity of 5’NT in the T cell fraction (p = 0.001) and in PBMC (p < 0.000). 5’NT activity was also lower in B cells, but this was not statistically significant. Enzyme activities did not correlate with indices of disease activity, disease duration or the B cell/T cell ratio and no influence of medication was found.

CONCLUSION Reduced lymphocyte 5’NT activity is a novel finding in SLE. These results indicate that purine metabolism in SLE may be disturbed. Consequences of a low 5’NT activity may be an intracellular accumulation of (deoxy)purine nucleotides and a reduction of adenosine production. It is hypothesised that these factors may play a part in the overall impaired immune function and in the chronicity of inflammation in SLE.

  • systemic lupus erythematosus
  • purine enzymes
  • immune function

https://doi.org/10.1136/ard.58.2.122

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