You are here

  • Home
  • Archive
  • Volume 57, Issue 1
  • Basic calcium phosphate crystals induce synthesis and secretion of 92 kDa gelatinase (gelatinase B/matrix metalloprotease 9) in human fibroblasts

Article Text

Article menu
Download PDFPDF
Concise reports
Basic calcium phosphate crystals induce synthesis and secretion of 92 kDa gelatinase (gelatinase B/matrix metalloprotease 9) in human fibroblasts
  1. Geraldine M McCarthya,
  2. Allison M Maciusa,
  3. Pamela A Christophersona,
  4. Lawrence M Ryana,
  5. Tayebeh Pourmotabbedb
  1. aDepartment of Medicine, Division of Rheumatology, Medical College of Wisconsin, Milwaukee, Wisconsin, bDepartment of Biochemistry, The University of Tennessee, Memphis, Tennessee
  1. Dr G M McCarthy, Division of Rheumatology, Froedtert Hospital, East 650, 9200 W Wisconsin Ave, Milwaukee, WI 53226, USA.

Abstract

OBJECTIVE Synovial fluid basic calcium phosphate (BCP) crystals are associated with severe joint degeneration accompanied by synovial hypertrophy. The metalloprotease 92 kDa gelatinase (MMP-9) has been implicated in the degradation of extracellular matrix in osteoarthritis, but the ability of BCP crystals to induce gelatinase in human fibroblasts or in adult porcine chondrocytes has not previously been studied. The hypothesis that the mitogenic response to BCP crystals is accompanied by induction and secretion of MMP-9 was studied.

METHODS MMP-9 messenger RNA (mRNA) was detected by northern blot and reverse transcription-polymerase chain reaction (RT-PCR). Gelatinase secretion was identified by western blot and zymography of conditioned media.

RESULTS BCP crystals caused a concentration dependent induction of MMP-9 mRNA accumulation and protein secretion in human fibroblasts but not in adult porcine chondrocytes.

CONCLUSION BCP crystals induce MMP-9 production by HF but not adult porcine chondrocytes. Fibroblast MMP-9 may be an important mediator of the joint destruction associated with synovial fluid BCP crystals.

  • gelatinase
  • crystals
  • osteoarthritis
  • fibroblasts

https://doi.org/10.1136/ard.57.1.56

Statistics from Altmetric.com

    Request Permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.

    View Full Text