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Cytokine production in whole blood cell cultures of patients with rheumatoid arthritis
  1. A J G Swaaka,b,
  2. H G van den Brinkb,
  3. L A Aardenb
  1. aDepartment of Rheumatology, Zuider Ziekenhuis, Rotterdam, the Netherlands, bDepartment of Auto Immune Diseases, Central Laboratory Red Cross Bloodtransfusion Service, Amsterdam, the Netherlands
  1. Dr A J G Swaak, Zuiderziekenhuis, Department of Rheumatology, Groene Hilledijk 315, Rotterdam, the Netherlands.

Abstract

OBJECTIVE The measurement of cytokine production of activated lymphocytes and monocytes in the whole blood cell (WBC) culture system may provide a sensitive tool for evaluating the actual ongoing immune response of patients with rheumatoid arthritis (RA).

METHODS Lipopolysaccharide (LPS) up to 250 pg/ml was used for the stimulation of monocytes for measuring the production of tumour necrosis factor α (TNFα), interleukin 6 (IL6) and IL12, while the anti-CD3 (1 μg/ml) and anti-CD28 (5 μg/ml) combination was used for T cell stimulation with the measuring of IL4 and interferon gamma (INFγ) production. Twenty seven patients with RA and 23 healthy controls were studied.

RESULTS The results showed a decreased IL6 (LPS stimulus 4–6 pg/ml) and IL12 (LPS stimulus 16–62 pg/ml) production in the RA patients. The maximal production of both cytokines was comparable with the normal controls. T cell stimulation showed a significant decreased INFγ production in the RA patients.

CONCLUSIONS These findings obtained in the WBC culture system are highly suggestive for a decreased TH-1 derived cytokine production by a diminished IL12 production in RA patients. Another possibility is that both IL12 and INFγ production in WBCs are inhibited by eventual circulating serum factors.

  • whole blood cell cultures
  • rheumatoid arthritis
  • cytokines

https://doi.org/10.1136/ard.56.11.693

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