Genus-and species-specific identification of mycoplasmas by 16S rRNA amplification

FJ Van Kuppeveld, JT Van der Logt… - Applied and …, 1992 - Am Soc Microbiol
Systematic computer alignment of mycoplasmal 16S rRNA sequences allowed the
identification of variable regions with both genus-and species-specific sequences. Species …

General primer-mediated polymerase chain reaction for detection of enteroviruses: application for diagnostic routine and persistent infections

GJ Zoll, WJ Melchers, H Kopecka… - Journal of clinical …, 1992 - Am Soc Microbiol
The aim of this study was to determine the applicability of the polymerase chain reaction
(PCR) for routine diagnostic use and for the detection of persistent enteroviral infections. To …

Detection of mycoplasma contamination in cell cultures by a mycoplasma group-specific PCR

FJ Van Kuppeveld, KE Johansson… - Applied and …, 1994 - Am Soc Microbiol
The suitability of a 16S rRNA-based mycoplasma group-specific PCR for the detection of
mycoplasma contamination in cell cultures was investigated. A total of 104 cell cultures were …

Kissing of the two predominant hairpin loops in the coxsackie B virus 3'untranslated region is the essential structural feature of the origin of replication required for …

WJ Melchers, JG Hoenderop, HJ Bruins Slot… - Journal of …, 1997 - Am Soc Microbiol
Higher-order RNA structures in the 3'untranslated region (3'UTR) of enteroviruses are
thought to play a pivotal role in viral negative-strand RNA synthesis. The structure of the …

General primer-mediated PCR for detection of Aspergillus species

WJ Melchers, PE Verweij, P Van den Hurk… - Journal of clinical …, 1994 - Am Soc Microbiol
A PCR assay was developed for the diagnosis of invasive aspergillosis in
immunocompromised patients. For this purpose, the complete nucleotide sequences of the …

[HTML][HTML] The coxsackievirus 2B protein increases efflux of ions from the endoplasmic reticulum and Golgi, thereby inhibiting protein trafficking through the Golgi

AS De Jong, HJ Visch, F De Mattia… - Journal of Biological …, 2006 - ASBMB
Coxsackievirus infection leads to a rapid reduction of the filling state of the endoplasmic
reticulum (ER) and Golgi Ca 2+ stores. The coxsackievirus 2B protein, a small membrane …

Direct PCR enables detection of Mycoplasma pneumoniae in patients with respiratory tract infections

JH Tjhie, FJ Van Kuppeveld… - Journal of clinical …, 1994 - Am Soc Microbiol
The sensitivities of three methods of detection of Mycoplasma pneumoniae by a 16S rDNA
PCR were compared by using a serial dilution of M. pneumoniae. These methods consisted …

Comparison of antigen detection and PCR assay using bronchoalveolar lavage fluid for diagnosing invasive pulmonary aspergillosis in patients receiving treatment …

PE Verweij, JP Latge, AJ Rijs… - Journal of clinical …, 1995 - Am Soc Microbiol
The performance of a sandwich enzyme-linked immunosorbent assay (ELISA) which detects
Aspergillus galactomannan (GM) was evaluated in bronchoalveolar lavage (BAL) fluid …

Coxsackie B3 virus protein 2B contains cationic amphipathic helix that is required for viral RNA replication

FJ Van Kuppeveld, JM Galama, J Zoll… - Journal of …, 1996 - Am Soc Microbiol
Enterovirus protein 2B has been shown to increase plasma membrane permeability. We
have identified a conserved putative amphipathic alpha-helix with a narrow hydrophilic face …

Cis‐element, oriR, involved in the initiation of (‐) strand poliovirus RNA: a quasi‐globular multi‐domain RNA structure maintained by tertiary ('kissing') interactions.

EV Pilipenko, KV Poperechny, SV Maslova… - The EMBO …, 1996 - embopress.org
The key steps in the replication of the poliovirus genome, initiation of (‐) and (+) strands,
require two different cis‐acting elements, oriR and oriL, respectively. It has been proposed …