Abstract
Adhesion molecules of the integrin family, including very late activation antigens (VLA), have been implicated in various cellular functions. In this study, we investigated the contribution of integrin-mediated interaction with ECM proteins to the cytokine gene expression in human chondrocytes. Human articular chondrocytes expressed VLA-1, -2, -3 and -5 on the cell surface, and could adhere to various ECM proteins, especially to fibronectin (FN). Furthermore, the production of GM-CSF and IL-6 was potently induced by culturing chondrocytes on immobilized FN. This stimulative effect of FN was completely inhibited by an anti-integrin alpha 5 chain mAb, as well as by anti-integrin beta 1 chain mAbs. These results indicate an important role of the VLA-5-mediated interaction with FN in regulating inflammatory cytokine production by human articular chondrocytes.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antibodies, Monoclonal / pharmacology
-
Cartilage, Articular / immunology*
-
Cartilage, Articular / metabolism
-
Cell Adhesion
-
Cells, Cultured
-
Collagen / pharmacology
-
Cytokines / biosynthesis*
-
Extracellular Matrix Proteins / pharmacology
-
Fibronectins / pharmacology*
-
Gene Expression
-
Granulocyte-Macrophage Colony-Stimulating Factor / biosynthesis
-
Humans
-
Integrins / analysis
-
Integrins / biosynthesis*
-
Interleukin-6 / biosynthesis
-
Macromolecular Substances
-
Mice
-
RNA, Messenger / analysis
-
RNA, Messenger / biosynthesis
-
Rats
-
Receptors, Fibronectin / immunology
-
Receptors, Fibronectin / physiology*
Substances
-
Antibodies, Monoclonal
-
Cytokines
-
Extracellular Matrix Proteins
-
Fibronectins
-
Integrins
-
Interleukin-6
-
Macromolecular Substances
-
RNA, Messenger
-
Receptors, Fibronectin
-
Granulocyte-Macrophage Colony-Stimulating Factor
-
Collagen