Association between reactive arthritis and antecedent infection with Shigella flexneri carrying a 2-Md plasmid and encoding an HLA-B27 mimetic epitope

Arthritis Rheum. 1993 Oct;36(10):1387-91. doi: 10.1002/art.1780361010.

Abstract

Objective: To determine whether Shigella flexneri strains that cause enteric infection and are associated with reactive arthritis (ReA) carry a 2-Md plasmid, pHS-2, which encodes an HLA-B27 mimetic epitope.

Methods: Plasmid DNA from Shigella isolates was characterized by DNA-DNA hybridization, restriction endonuclease digestion, and sequencing.

Results: S flexneri strains associated with ReA carried a 2-Md plasmid homologous to pHS-2.

Conclusion: The finding of pHS-2 in additional Shigella strains associated with ReA underscores its potential importance in the etiology of the disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arthritis, Reactive*
  • Base Sequence
  • DNA Restriction Enzymes
  • DNA, Bacterial / genetics
  • Dysentery, Bacillary / genetics*
  • Epitopes*
  • HLA-B27 Antigen / genetics*
  • HLA-B27 Antigen / immunology*
  • Humans
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Plasmids*
  • Prohibitins
  • Sequence Homology, Nucleic Acid
  • Shigella / genetics
  • Shigella flexneri / genetics*

Substances

  • DNA, Bacterial
  • Epitopes
  • HLA-B27 Antigen
  • PHB2 protein, human
  • Prohibitins
  • DNA Restriction Enzymes