Amplification of plasmid and chromosome Chlamydia DNA in synovial fluid of patients with reactive arthritis and undifferentiated seronegative oligoarthropathies

S Bas; R Griffais; T K Kvien; A Glennås; K Melby; T L Vischer

doi:10.1002/art.1780380718

Amplification of plasmid and chromosome Chlamydia DNA in synovial fluid of patients with reactive arthritis and undifferentiated seronegative oligoarthropathies

Arthritis Rheum. 1995 Jul;38(7):1005-13. doi: 10.1002/art.1780380718.

Authors

S Bas¹, R Griffais, T K Kvien, A Glennås, K Melby, T L Vischer

Affiliation

¹ Research Laboratory, University Hospital, Geneva, Switzerland.

PMID: 7612032
DOI: 10.1002/art.1780380718

Abstract

Objective: To investigate the hypothesis that whole bacteria might be found in the joints of patients with Chlamydia-associated reactive arthritis.

Methods: The presence of 2 plasmid- and 2 chromosome-specific sequences of Chlamydia DNA was investigated by amplification with the polymerase chain reaction, in synovial fluid (SF) samples from 71 patients with various arthropathies.

Results: Chlamydia DNA was found in SF samples from 22 patients.

Conclusion: Whole chlamydiae are likely present in the SF of patients with Chlamydia-associated reactive arthritis.

MeSH terms

Adolescent
Adult
Arthritis, Reactive / microbiology*
Base Sequence
Blotting, Southern
Chlamydia / genetics*
Chlamydia / isolation & purification
Chlamydia Infections / microbiology*
Chromosomes, Bacterial / chemistry
DNA, Bacterial / analysis*
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Female
Gene Amplification
Humans
Male
Middle Aged
Molecular Sequence Data
Plasmids / genetics
Polymerase Chain Reaction
Sensitivity and Specificity
Synovial Fluid / microbiology*

Substances

DNA, Bacterial