Abstract
Objective:
To examine the de novo synthesis and cellular distribution of the E-selectin adhesion molecule in synovial tissues obtained from patients with rheumatoid arthritis (RA).
Methods:
Immunohistochemistry techniques combined with in situ hybridization were used to examine RA synovium.
Results:
There were numerous endothelial cells positive for E-selectin and E-selectin messenger RNA in the RA synovial membranes. Moreover, E-selectin expression appeared to correlate with inflammatory activity.
Conclusion:
The strong vascular expression of E-selectin indicates an activation of endothelial cells in the recruitment of cells associated with the chronic inflammation of RA.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Arthritis, Rheumatoid / genetics
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Arthritis, Rheumatoid / metabolism*
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Base Sequence
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Cell Adhesion Molecules / analysis*
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Cell Adhesion Molecules / biosynthesis
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Cell Adhesion Molecules / genetics*
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DNA Primers / analysis
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DNA Primers / chemistry
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DNA Primers / genetics
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E-Selectin
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Female
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Humans
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Immunohistochemistry
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In Situ Hybridization
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Male
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Middle Aged
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Molecular Sequence Data
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RNA, Messenger / analysis*
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Synovial Membrane / chemistry
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Synovial Membrane / metabolism
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Up-Regulation
Substances
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Cell Adhesion Molecules
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DNA Primers
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E-Selectin
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RNA, Messenger