Abstract
Interleukin-1 (IL-1) treatment of synovial cells from rheumatoid arthritis and osteoarthritis patients resulted in a dose-dependent secretion of phospholipase A2 (PLA2). IL-1 also stimulated prostaglandin E2 and plasminogen activator synthesis, in parallel with PLA2 activation; all 3 were detectable within 6 hours of IL-1 treatment and peaked by 24 hours. Synovial cell PLA2 required calcium (5 mM) and a neutral pH (7.5) for maximal activity and appears similar to the PLA2 in synovial fluid, which has been described previously. We conclude that PLA2 can be induced by IL-1, and its secretion may contribute significantly to the inflammatory actions of IL-1.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arthritis, Rheumatoid / enzymology
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Arthritis, Rheumatoid / metabolism
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Arthritis, Rheumatoid / pathology
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Calcium / pharmacology
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Dinoprostone
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Enzyme Activation
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Humans
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Hydrogen-Ion Concentration
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Interleukin-1 / pharmacology*
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Kinetics
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Osteoarthritis / enzymology
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Osteoarthritis / metabolism
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Osteoarthritis / pathology
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Phospholipases / metabolism*
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Phospholipases A / metabolism*
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Phospholipases A2
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Plasminogen Activators / metabolism
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Prostaglandins E / metabolism
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Synovial Membrane / enzymology*
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Synovial Membrane / metabolism
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Synovial Membrane / pathology
Substances
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Interleukin-1
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Prostaglandins E
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Phospholipases
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Phospholipases A
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Phospholipases A2
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Plasminogen Activators
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Dinoprostone
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Calcium