Abstract
A plan for DRB typing at the sequence level is detailed. Only one polymerase chain amplification reaction is needed and the application of a limited number of short oligonucleotide probes allows an almost complete definition of DRB alleles. The scheme was tested on 40 homozygous cell-lines selected to cover a wide range of specificities, and 40 RFLP-typed controls. The results are presented and discussed. The simplicity and accuracy of this scheme are emphasized.
MeSH terms
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Alleles
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Autoradiography
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Base Sequence
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DNA / analysis
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DNA / genetics
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HLA-DR Antigens / genetics
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HLA-DR Antigens / immunology*
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Heterozygote
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Histocompatibility Antigens Class II / genetics
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Histocompatibility Antigens Class II / immunology*
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Histocompatibility Testing / methods*
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Homozygote
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Humans
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Molecular Sequence Data
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Nucleic Acid Hybridization
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Oligonucleotide Probes*
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Polymerase Chain Reaction
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Polymorphism, Genetic
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Polymorphism, Restriction Fragment Length
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Quaternary Ammonium Compounds
Substances
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HLA-DR Antigens
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Histocompatibility Antigens Class II
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Oligonucleotide Probes
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Quaternary Ammonium Compounds
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DNA
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tetramethylammonium