Abstract
Beta-glucans are structural components of fungal cell walls which are involved in the immune recognition of fungal pathogens and possess beneficial immunomodulatory activities in isolated form. Here we have developed a soluble chimeric form of the major mammalian beta-glucan receptor, Dectin-1, and demonstrate its application for the detection and characterisation of soluble and insoluble beta-glucans, including fungal particles, using ELISA, flow cytometric and fluorescence-based microscopy assays.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibody Specificity*
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Cell Line
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Cell Wall / metabolism
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Enzyme-Linked Immunosorbent Assay / methods*
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Flow Cytometry
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Fungal Proteins / analysis*
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Glucans / chemistry
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Humans
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Lectins, C-Type
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Membrane Proteins / analysis*
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Microscopy, Fluorescence
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Nerve Tissue Proteins / analysis*
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Recombinant Fusion Proteins / chemistry
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Saccharomyces cerevisiae*
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Solubility
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beta-Glucans / analysis*
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beta-Glucans / chemistry
Substances
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Fungal Proteins
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Glucans
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Lectins, C-Type
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Membrane Proteins
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Nerve Tissue Proteins
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Recombinant Fusion Proteins
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beta-Glucans
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dectin 1
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glucan phosphate