Galectin-induced activation of the transcription factors NFAT and AP-1 in human Jurkat T-lymphocytes

Cell Signal. 2002 Oct;14(10):861-8. doi: 10.1016/s0898-6568(02)00035-9.

Abstract

Galectin-mediated ligation of glycoepitopes on T-cell activation markers induces an increase in the cytosolic calcium concentration ([Ca(2+)](i)) originating from a transient Ca(2+) release of internal stores as well as a sustained influx across the plasma membrane. In transiently transfected Jurkat T-lymphocytes, galectins [galectin-1 (gal-1), recombinant human galectin-1 (rec gal-1), nematode 32-kDa galectin (LEC-1), nematode 16-kDa galectin (LEC-6)] differentially stimulate the expression of the luciferase reporter gene constructs pNFAT-TA-Luc and pAP1(PMA)-TA-Luc, which are activated by the nuclear factor of activated T-cells (NFAT) or the transcription factor, activator protein 1 (AP-1), respectively. The galectin-stimulated expression of the reporter constructs is completely inhibited by lactose (30 mM) and asialofetuin (30 microM) carrying Galbeta1-4GlcNAc sequences. Preincubation of pNFAT-TA-Luc-transfected cells with cyclosporine A (0.1 microM) and FK506 (0.01 microM) abrogated the gal-1-induced expression of the reporter luciferase (Luc). Electrophoretic mobility shift assays (EMSAs) provided evidence for gal-1-stimulated increase in the binding of nuclear extracts to a synthetic oligonucleotide with an AP-1 consensus sequence.

MeSH terms

  • Asialoglycoproteins / pharmacology
  • Binding Sites / drug effects
  • Binding Sites / genetics
  • Binding Sites / immunology
  • Calcium Signaling / drug effects
  • Calcium Signaling / genetics
  • Calcium Signaling / immunology*
  • Cell Membrane / drug effects
  • Cell Membrane / immunology*
  • Cell Membrane / metabolism
  • Cyclosporine / pharmacology
  • Cytosol / drug effects
  • Cytosol / immunology*
  • Cytosol / metabolism
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Female
  • Fetuins
  • Galectin 1 / genetics
  • Galectin 1 / immunology*
  • Galectin 1 / metabolism
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / immunology
  • Genes, Reporter / genetics
  • Genes, Reporter / immunology
  • Humans
  • Jurkat Cells
  • Lactose / pharmacology
  • Lymphocyte Activation / drug effects
  • Lymphocyte Activation / immunology*
  • NFATC Transcription Factors
  • Nuclear Proteins*
  • Oligonucleotide Probes
  • Pancreatitis-Associated Proteins
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / immunology
  • Recombinant Fusion Proteins / metabolism
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / metabolism
  • Tacrolimus / pharmacology
  • Transcription Factor AP-1 / genetics
  • Transcription Factor AP-1 / immunology
  • Transcription Factor AP-1 / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • alpha-Fetoproteins / pharmacology

Substances

  • Asialoglycoproteins
  • DNA-Binding Proteins
  • Fetuins
  • Galectin 1
  • NFATC Transcription Factors
  • Nuclear Proteins
  • Oligonucleotide Probes
  • Pancreatitis-Associated Proteins
  • REG3A protein, human
  • Recombinant Fusion Proteins
  • Transcription Factor AP-1
  • Transcription Factors
  • alpha-Fetoproteins
  • asialofetuin
  • Cyclosporine
  • Lactose
  • Tacrolimus