Abstract
Oncostatin M in combination with interleukin-1 (IL-1) induced a rapid and reproducible release of collagen from bovine nasal cartilage in culture. This release was accompanied by a high collagenolytic activity and low or absent tissue inhibitor of metalloproteinase-1 activity in the culture medium. Transforming growth factor-beta1 was able to block this release of collagen from the tissue, and reduce the expression and secretion of collagenases whilst maintaining TIMP-1 levels from bovine nasal chondrocytes. This study shows for the first time that TGF-beta1 can protect cartilage collagen from destruction.
Copyright 2000 Academic Press.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cattle
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Cells, Cultured
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Collagen / metabolism*
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Collagenases / genetics*
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Collagenases / metabolism
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Growth Inhibitors / pharmacology
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Interleukin-1 / pharmacology*
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Matrix Metalloproteinase 1 / genetics*
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Matrix Metalloproteinase 1 / metabolism
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Matrix Metalloproteinase 13
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Nasal Septum / cytology
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Nasal Septum / drug effects
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Nasal Septum / metabolism*
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Oncostatin M
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Peptide Fragments / metabolism
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Peptides / pharmacology*
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RNA, Messenger / genetics
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Tissue Inhibitor of Metalloproteinase-1 / genetics*
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Tissue Inhibitor of Metalloproteinase-1 / metabolism
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Transforming Growth Factor beta / pharmacology*
Substances
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Growth Inhibitors
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Interleukin-1
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Peptide Fragments
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Peptides
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RNA, Messenger
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Tissue Inhibitor of Metalloproteinase-1
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Transforming Growth Factor beta
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Oncostatin M
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Collagen
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Collagenases
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Matrix Metalloproteinase 13
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Matrix Metalloproteinase 1