Transforming growth factor beta1 blocks the release of collagen fragments from boving nasal cartilage stimulated by oncostatin M in combination with IL-1alpha

Cytokine. 2000 Jun;12(6):765-9. doi: 10.1006/cyto.1999.0625.

Abstract

Oncostatin M in combination with interleukin-1 (IL-1) induced a rapid and reproducible release of collagen from bovine nasal cartilage in culture. This release was accompanied by a high collagenolytic activity and low or absent tissue inhibitor of metalloproteinase-1 activity in the culture medium. Transforming growth factor-beta1 was able to block this release of collagen from the tissue, and reduce the expression and secretion of collagenases whilst maintaining TIMP-1 levels from bovine nasal chondrocytes. This study shows for the first time that TGF-beta1 can protect cartilage collagen from destruction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Cells, Cultured
  • Collagen / metabolism*
  • Collagenases / genetics*
  • Collagenases / metabolism
  • Growth Inhibitors / pharmacology
  • Interleukin-1 / pharmacology*
  • Matrix Metalloproteinase 1 / genetics*
  • Matrix Metalloproteinase 1 / metabolism
  • Matrix Metalloproteinase 13
  • Nasal Septum / cytology
  • Nasal Septum / drug effects
  • Nasal Septum / metabolism*
  • Oncostatin M
  • Peptide Fragments / metabolism
  • Peptides / pharmacology*
  • RNA, Messenger / genetics
  • Tissue Inhibitor of Metalloproteinase-1 / genetics*
  • Tissue Inhibitor of Metalloproteinase-1 / metabolism
  • Transforming Growth Factor beta / pharmacology*

Substances

  • Growth Inhibitors
  • Interleukin-1
  • Peptide Fragments
  • Peptides
  • RNA, Messenger
  • Tissue Inhibitor of Metalloproteinase-1
  • Transforming Growth Factor beta
  • Oncostatin M
  • Collagen
  • Collagenases
  • Matrix Metalloproteinase 13
  • Matrix Metalloproteinase 1