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Growth Factor, Cytokines and Cell Signaling

Expression and responsiveness of human interleukin-18 receptor (IL-18R) on hematopoietic cell lines

Leukemia volume 14, pages 1052–1059 (2000)Cite this article

Abstract

Interleukin-18 (IL-18) is a new inflammatory cytokine sharing biological functions with IL-12. The human IL-18 receptor (IL-18R) was recently identified and was found to be expressed on normal peripheral blood lymphocytes. To further characterize IL-18R, we analyzed IL-18R expression using a series of human hematopoietic cell lines selected from various cell lineages. We found the IL-18R expression on cells of T and B lineages as expected from analysis on normal cells. The IL-18R expression, however, was found not to be restricted to any specific maturation stages of T and B cells. In addition, we detected IL-18R expression in myeloid, monocytoid, erythroid and megakaryocytic cell lines, indicating that normal counterparts of these cell lineages could express IL-18R and participate in in vivo reactions caused by IL-18. Biochemical studies showed that IL-18R proteins exist as heterogeneous molecules ranging from 60 to 110 kDa. Deglycosilation experiments indicated that the heterogeneity could not be explained only by a difference in glycosilation. We also found that tumor necrosis factor-α (TNF-α) modulated the IL-18R expression, which implies an important in vivo effect of TNF-α on IL-18-induced reaction. Analyzing the responsiveness of IL-18R, we found that only KG-1 responded to IL-18 stimulation. This suggests that certain inhibitory mechanisms of IL-18 responsive genes are involved in the all IL-18R-positive cell lines except KG-1.

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Acknowledgements

We thank Drs Hans G Drexler (DSMZ-German Collection of Microorganisms and Cell Cultures), Michiyuki Maeda (Kyoto University), Eiji Tatsumi (Kobe University), Kimitaka Sagawa (Kurume University), Jun Okamura (Kyushu Cancer Center) and Takemi Otsuki (Kawasaki Medical School) for providing some of the leukemia cell lines used in this study. We also thank Dr Mark Micallef for helpful discussion and Ms Keleher for editing the manuscript.

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Authors and Affiliations

  1. Fujisaki Cell Center, Hayashibara Biochemical Laboratories, Inc., Okayama, Japan

    S Nakamura, T Otani, R Okura, Y Ijiri, R Motoda & K Orita

  2. Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc., Okayama, Japan

    M Kurimoto

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Nakamura, S., Otani, T., Okura, R. et al. Expression and responsiveness of human interleukin-18 receptor (IL-18R) on hematopoietic cell lines. Leukemia 14, 1052–1059 (2000). https://doi.org/10.1038/sj.leu.2401789

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