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Angiogenesis mediated by soluble forms of E-selectin and vascular cell adhesion molecule-1

Abstract

ENDOTHELIAL adhesion molecules facilitate the entry of leukocytes into inflamed tissues. This in turn promotes neovascularization, a process central to the progression of rheumatoid arthritis, tumour growth and wound repair1. Here we test the hypothesis that soluble endothelial adhesion molecules promote angiogenesis2á¤-4. Human recombinant soluble E-selectin and soluble vascular cell adhesion molecule-1 induced chemotaxis of human endothelial cells in vitro and were angiogenic in rat cornea. Soluble E-selectin acted on endothelial cells in part through a sialyl Lewis-X-dependent mechanism, while soluble vascular cell adhesion molecule-1 acted on endothelial cells in part through a very late antigen (VLA)-4 dependent mechanism. The chemotactic activity of rheumatoid synovial fluid for endothelial cells, and also its angiogenic activity, were blocked by antibodies to either soluble E-selectin or soluble vascular cell adhesion molecule-1. These results suggest a novel function for soluble endothelial adhesion molecules as mediators of angiogenesis.

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References

  1. Folkman, J. & Shing, Y. J. biol. Chem. 267, 10931–10934 (1992).

    CAS  PubMed  Google Scholar 

  2. Lobb, R. R. et al. J. Immun. 147, 124–129 (1991).

    CAS  PubMed  Google Scholar 

  3. Lo, S. K. et al. J. exp. Med. 173, 1493–1500 (1991).

    Article  CAS  Google Scholar 

  4. Pober, J. S. et al. J. Immun. 136, 1680–1687 (1986).

    CAS  PubMed  Google Scholar 

  5. Koch, A. E. et al. Science 258, 1798–1801 (1992).

    Article  ADS  CAS  Google Scholar 

  6. Munro, J. M. et al. Am. J. Path. 141, 1397–1408 (1992).

    CAS  PubMed  Google Scholar 

  7. Paavonen, T. & Renkonen, R. Am. J. Path. 141, 1259–1264 (1992).

    CAS  PubMed  Google Scholar 

  8. Gillard, B. K., Jones, M. A. & Marcus, D. M. Archs Biochem. Biophys. 256, 435–445 (1987).

    Article  CAS  Google Scholar 

  9. Massia, S. P. & Hubbell, J. A. J. biol. Chem. 267, 14109–14206 (1992).

    Google Scholar 

  10. Johnson, B. A., Haines, G. K., Harlow, L. A. & Koch, A. E. Arthritis Rheum. 36, 137–146 (1993).

    Article  CAS  Google Scholar 

  11. Brooks, P. C., Clark, R. A. & Cheresh, D. A. Science 264, 569–571 (1994).

    Article  ADS  CAS  Google Scholar 

  12. Gamble, J. R. et al. J. Cell Biol. 121, 931–943 (1993).

    Article  CAS  Google Scholar 

  13. Nguyen, M., Strubel, N. A. & Bischoff, J. Nature 365, 267–269 (1993).

    Article  ADS  CAS  Google Scholar 

  14. Koch, A. E. et al. Lab. Invest. 64, 313–320 (1991).

    CAS  PubMed  Google Scholar 

  15. Koch, A. E., Turkiewicz, W., Harlow, L. A. & Pope, R. M. Clin. Immun. Immunopatn. 69, 29–35 (1993).

    Article  CAS  Google Scholar 

  16. Carson, C. W., Beall, L. D., Hunder, G. G., Johnson, C. M. & Newman, W. J. Rheumatol. 21, 605–611 (1994).

    CAS  PubMed  Google Scholar 

  17. Koch, A. E. et al. J. Immun. 152, 149–152 (1994).

    Google Scholar 

  18. Klagsbrun, M. & D'Amore, P. A. A. Rev. Physiol. 53, 217–239 (1991).

    Article  CAS  Google Scholar 

  19. Leibovich, S. J. et al. Nature 329, 630–632 (1987).

    Article  ADS  CAS  Google Scholar 

  20. Bussolino, F. et al. Nature 337, 471–473 (1989).

    Article  ADS  CAS  Google Scholar 

  21. Leibovich, S. J., Polverini, P. J., Fong, T. W., Harlow, L. A. & Koch, A. E. Proc. natn. Acad. Sci. U.S.A 91, 4190–4194 (1994).

    Article  ADS  CAS  Google Scholar 

  22. Carlos, T. M. et al. Blood 76, 965–970 (1990).

    CAS  PubMed  Google Scholar 

  23. Osborn, L., Vassolla, C. & Benjamin, C. D. J. exp. Med. 176, 99–107 (1992).

    Article  CAS  Google Scholar 

  24. Pulido, R. et al. J. biol. Chem. 266, 10241–10245 (1991).

    CAS  PubMed  Google Scholar 

  25. Winer, B. J. Statistical Principles in Experimental Design (McGraw-Hill, New York, 1971).

    Google Scholar 

Download references

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Koch, A., Halloran, M., Haskell, C. et al. Angiogenesis mediated by soluble forms of E-selectin and vascular cell adhesion molecule-1. Nature 376, 517–519 (1995). https://doi.org/10.1038/376517a0

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