Abstract
We have previously shown1,2 that a serum raised against certain isolates of Klebsiella pneumoniae lyses the lymphocytes of HLA-B27-positive patients with ankylosing spondylitis (AS) but not of B27-positive or B27-negative healthy controls. These observations suggested that some Klebsiella antigens cross-react with a gene product intimately associated with B27 or possibly with ‘modified’ B27 (refs 1, 3) in patients with AS. It seems likely therefore, that some Klebsiella antigens play a role in the pathogenesis of AS, perhaps by specifically modifying a B27-associated cell-surface marker. We have now examined the influence of culture filtrates from three Klebsiella isolates on lymphocytes from B27-positive and B27-negative healthy individuals. We report that 24-h culture filtrates of Klebsiella K43 (previously Klebsiella F19) contain a factor(s) capable of specifically modifying the sensitivity to anti-Klebsiella K43 serum of B27+AS− lymphocytes (that is, cells not lysed by anti-Klebsiella K43 serum): ‘modified’ B27+AS− lymphocytes are now serologically similar to the cells of B27-positive patients with AS (B27+AS+). The failure of anti-Klebsiella F10 and of anti-Escherichia coli sera to lyse lymphocytes, which have been incubated with the corresponding culture filtrates, excludes a nonspecific lysis by the anti-bacterial sera of target lymphocytes bearing bacterial antigens randomly distributed on their surface. Therefore, the data are compatible with a specific transformation by a Klebsiella K43-derived soluble factor of a B27-associated lymphoid cell component.
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References
Seager, K., Bashir, H. V., Geczy, A. F., Edmonds, J. & de Vere-Tyndall, A. Nature 277, 68–70 (1979).
Geczy, A. F. & Yap, J. Lancet i, 719–720 (1979).
Geczy, A. F., Seager, K., Bashir, H. V., de Vere-Tyndall, A. & Edmonds, J. J. clin. Lab. Immun. (in the press).
Bennett, P. & Wood, P. (eds) Population Studies of the Rheumatic Diseases, Proc. 3rd Int. Symp., 456; 478 (Excerpta Medica, New York, 1966).
Petit, J. C. & Unanue, E. R. J. Immun. 113, 984–992 (1974).
Cohen, J. J., Rodriguez, G. E., Kind, P. D. & Campbell, P. A. J. Immun. 114, 1132–1134 (1975).
Campbell, P. A., Schuffler, C. & Rodriguez, G. E. J. Immun. 116, 590–594 (1976).
Campbell, P. A., Rodriguez, G. E. & Schuffler, C. Cell Immun. 17, 418–422 (1975).
Rodriguez, G. E., McClatchy, J. K. & Campbell, P. A. Infect. Immun. 10, 1163–1169 (1974).
Gronowicz, E. & Coutinho, A. Transplantn Rev. 24, 3–40 (1975).
Damais, C. et al. J. Immun. 115, 268–271 (1975).
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Geczy, A., Alexander, K., Bashir, H. et al. A factor(s) in Klebsiella culture filtrates specifically modifies an HLA-B27-associated cell-surface component. Nature 283, 782–784 (1980). https://doi.org/10.1038/283782a0
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DOI: https://doi.org/10.1038/283782a0
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