Mechanisms of allergy and clinical immunologyCytometry by time-of-flight immunophenotyping identifies a blood Sjögren's signature correlating with disease activity and glandular inflammation
Graphical abstract
Section snippets
Study participants
All patients with pSS (n = 49) met the American European Consensus criteria (AECG) diagnostic criteria.20 Patients were referred to the Department of Rheumatology of Hôpitaux Universitaires Paris-Sud (France) between December 2013 and September 2014. Control subjects (n = 45) were either healthy volunteers or patients with sicca syndrome with a negative diagnostic result for pSS. Clinical evaluation confirmed that the latter had neither autoantibodies nor a positive biopsy result. The study was
Identification of a cellular signature in peripheral blood from patients with pSS
The correlation between flow and mass cytometry was very good in PBMCs from 9 healthy donors (average R2 = 0.92 ± 0.09), which confirmed the validity of mass cytometry for clinical immunophenotyping (see Fig E1 in this article's Online Repository at www.jacionline.org). Characteristics of the 49 patients with pSS and 45 control subjects are summarized in Table E3 in this article's Online Repository at www.jacionline.org. Using the viSNE algorithm,21 we identified 40 peripheral blood cell types
Discussion
This study is the first large-scale clinical application of mass cytometry to stratify a patient population with a chronic disease. In addition, this is also the first study that performs immunophenotyping of paired blood and biopsy samples to correlate cellular changes in the blood and target tissue of the disease. The strength of this high-dimensional and unbiased immunophenotyping approach relies on its ability to identify new cellular variations and, importantly, to enable the simultaneous
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Cited by (0)
Supported by an unrestricted grant (UPSud/SAIC N 97731) from Biogen to Université Paris-Sud.
Disclosure of potential conflict of interest: M. Mingueneau is an employee of Biogen. S. Boudaoud received a grant from Biogen and is an employee of INSERM. S. Haskett is an employee of Biogen. T. L. Reynolds is an employee of Biogen. G. Nocturne has received a grant from Biogen. E. Norton is an employee of Biogen. X. Zhang is an employee of Biogen. M. Constant is an employee of Biogen. D. Park is an employee of Biogen. W. Wang is an employee and holds stock in Biogen. T. Lazure is an employee of Assitance Publique Hopitaux de Paris. A. Ergun is an employee of Biogen. X. Mariette received a grant from Biogen and provided consultancy for Biogen. The rest of the authors declare that they have no relevant conflicts of interest.
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These authors contributed equally to this work.
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These authors contributed equally to this work.