Influence of cytostatic agents on the pulmonary defence of mice infected with Klebsiella pneumoniae and on the efficacy of treatment with ceftriaxone

doi:10.1016/S0163-4453(94)95087-3

Journal of Infection

Volume 29, Issue 1, July 1994, Pages 53-66

https://doi.org/10.1016/S0163-4453(94)95087-3 Get rights and content

Summary

The effect of cytostatic treatment on the cellular defence and the efficacy of treatment with ceftriaxone in Klebsiella pneumoniae pneumonia was studied. Mice, made monocytopenic and granulocytopenic by cyclophosphamide or monocytopenic by etoposide, were infected intratracheally with K. pneumoniae (approximately 10⁴ CFU) and then treated with ceftriaxone. At various intervals, the numbers of bacteria in the broncho-alveolar lavage (BAL) fluid and in lungs homogenised after lavage were determined. Cyclophosphamide reduced the numbers of granulocytes in the BAL fluid significantly but reduced only slightly the number of alveolar macrophages at the time of inoculation, 12 and 15 h later. The number of CFU in cyclophosphamide-treated mice was higher than that in controls, being significant in the homogenised lungs at 15 h after infection. In etoposide-treated mice, the numbers of alveolar phagocytes in BAL did not differ from those in control mice, whereas the number of bacteria was lower (only significantly in BAL fluid at 15 h after infection) than that in the controls. In this short experimental infection cytostatic treatment did not affect the outgrowth of Klebsiella pneumoniae substantially or the efficacy of treatment with ceftriaxone.

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Citation Excerpt :
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Objectives: The effect of cyclophosphamide-induced leukocytopenia on the cellular defence and on the efficacy of penicillin treatment in a Streptococcus pneumoniae pneumonia model in mice was studied.
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2006, FEMS Immunology and Medical Microbiology

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Original articleInfluence of cytostatic agents on the pulmonary defence of mice infected with Klebsiella pneumoniae and on the efficacy of treatment with ceftriaxone

Summary

Am J Med

J Immunol Methods

J Immunol Methods

Chemotherapy of neoplastic diseases

Host defense mechanisms and their modification by cancer therapy

Methods Cancer Res

Infection complicating bone marrow transplantation

Pulmonary bacterial infections in the immunocompromised host

Semin Resp Med

Epidemiology and prevention of infection in the compromised host

Evaluation of ceftazidime in experimental Klebsiella pneumoniae pneumonia: comparison with other antibiotics and measurement of its penetration into respiratory tissues and secretions

J Antimicrob Chemother

Empiric treatment of infections in neutropenic patients with cancer

Rev Infect Dis

The problem of neutropenia resulting from cancer therapy

Clinician

Influence of etoposide and cyclophosphamide on the efficacy of cloxacillin and erythromycin in an experimental staphylococcal infection

Antimicrob Agents Chemother

Lung defenses against viral and bacterial challenges during immunosuppression with cyclophosphamide in mice

Am Rev Respir Dis

Studies of lung infections caused by Pseudomonas aeruginosa in mice treated with cyclophosphamide

Infection

Quantitative effect of granulocytes on antibiotic treatment of experimental Staphylococcal infection

Antimicrob Agents Chemother

Quantitative determination of the effect of granulocytes on the course of experimental infections during antibiotic treatment

Infection

Role of granulocytes and monocytes in experimental Staphylococcus epidermidis endocarditis

Infect Immun

Effect of irradiation, cyclophosphamide and etoposide (VP-16) on the number of peripheral blood and peritoneal leucocytes in mice under normal conditions and during an acute inflammatory reaction

Inflammation

The macrophage

Origin, kinetics and characteristics of pulmonary macrophages in the normal steady state

J Exp Med

Original article
Influence of cytostatic agents on the pulmonary defence of mice infected with Klebsiella pneumoniae and on the efficacy of treatment with ceftriaxone