Elsevier

Human Immunology

Volume 21, Issue 2, February 1988, Pages 115-124
Human Immunology

Association between HLA phenotype and HLA concentration in plasma or platelets

https://doi.org/10.1016/0198-8859(88)90086-9Get rights and content

Abstract

To understand the relationship between HLA phenotype and plasma or platelet HLA better, concentrations of plasma and platelet HLA were measured in 215 individuals of known HLA phenotypes. Precise quantitation of HLA antigens was achieved by means of an enzyme-linked immunoassay using the W6/32 monoclonal antibody and purified HLA molecules. The mean plasma and platelet HLA concentrations were 2.04 ± 1.67 μg/mlSD, n = 215) and 11.28 ± 4.65 fg/cellSD, n = 213), respectively. Statistical analysis of associations between HLA phenotypes and plasma HLA revealed that the mean plasma HLA concentration of individuals with HLA-A23 or HLA-A24 was 1.4 (p < 0.002) or 1.9 (p < 0.001) times higher than those without these two HLA antigens. Furthermore, the mean plasma HLA concentration of individuals who have HLA-A26 was 25% less than those without HLA-A26 (p < 0.05). In contrast, the only association between HLA phenotypes and HLA concentrations of platelets was observed in HLA-B7-positive individuals. The mean platelet HLA concentration of HLA-B7 individuals was 27% higher than those without HLA-B7 (p < 0.005). This finding is in accordance with previous observations made on red blood cells. The results indicate that the HLA concentrations in plasma are regulated, at least in part, by genetic factors that are different from those regulating platelet HLA.

References (27)

  • J.J. van Rood et al.

    Anti-HL-A9 inhibitor in normal human serum

    Nature

    (1970)
  • M.A. Pellegrino et al.

    Evaluation of two sources of soluble HLA antigens: Platelets and serum

    Eur J Immunol

    (1974)
  • P.A. Thomasulo

    Management of the alloimmunized patient with LA-matched platelets

  • Cited by (0)

    View full text