Establishment of an immature mast cell line from a patient with mast cell leukemia

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Abstract

A cell line showing many characteristics of immature mast cells has been established from the peripheral blood of a patient with mast cell leukemia. Cultured cells contain low levels of histamine, are stained metachromatically by toluidine blue, and contain chloroacetate esterase, aminocaproate esterase and tryptase activities. The cells lack T and B lymphocyte, as well as myeloid cell markers, and do not possess IgE receptors. Solid tumors of metachromatically positive cells have been successfully induced and serially passed in nude mice using 5-azacytidine transformed cells. This cell line may be useful for future studies of mast cells and their constituents.

References (60)

  • J.M. Johnstone

    The appearance and significance of tissue mast cells in human bone marrow

    J. clin. Path.

    (1954)
  • J.H. McCarthy et al.

    The presence of mast cells in agar cultures

    Expl Hemat.

    (1980)
  • T. Sun et al.

    Atlas of Cytochemistry and Immunochemistry of Hematologic Neoplasms

  • S. Strobel et al.

    Human intestinal mucosal mast cells: evaluation of fixation and staining techniques

    J. clin. Path.

    (1981)
  • A.G.E. Pearse
  • D. Metcalf et al.

    Detection of a new type of mouse eosinophil colony by luxol-fast-blue staining

    Expl Hemat.

    (1980)
  • T. Sun et al.

    Atlas of Cytochemistry and Immunochemistry of Hematologic Neoplasms

  • T. Sun et al.

    Atlas of Cytochemistry and Immunochemistry of Hematologic Neoplasms

  • S.J. Ackerman et al.

    Formation of Charcot-Leyden crystals by human basophils

    J. exp. Med.

    (1982)
  • M.D. Tharp et al.

    Conjugated avidin binds to mast cell granules

    J. Histochem. Cytochem.

    (1985)
  • A.A. Irani et al.

    Two types of human mast cells that have distinct neutral protease compositions

  • G.W. Dewald et al.

    Cytogenetic diagnosis of malignant pleural effusions. Culture methods to supplement direct preparations in diagnosis

  • G.W. Dewald

    Chromosome study of autopsy tissue

  • C.-Y. Li et al.

    Practical immunocytochemical identification of human blood cells

    Am. J. clin. Path.

    (1984)
  • T. Sun et al.

    Atlas of Cytochemistry and Immunochemistry of Hematologic Neoplasms

  • B. Reedman et al.

    Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and nonproducer lymphoblastoid cell lines

    Int. J. Cancer

    (1973)
  • D.L. Wassom et al.

    Elevated serum levels of the eosinophil granule major basic protein in patients with eosinophilia

    J. clin. Invest.

    (1981)
  • S.J. Ackerman et al.

    Distinctive cationic proteins of the human eosinophil granule: major basic protein, eosinophil cationic protein, and eosinophil-derived neurotoxin

    J. Immun.

    (1983)
  • L.H. Peterson et al.

    Fine specificity, structure and proteolytic susceptibility of the human lymphocyte receptor for IgE

    J. Immun.

    (1985)
  • A. Gonzalez-Molina et al.

    A subpopulation of normal human peripheral blood B lymphocytes that bind IgE

    J. clin. Invest.

    (1977)
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    Supported in part by grants from the National Institutes of Health, AI 20416, AI 15231 and AI 09728, and by the Mayo Foundation.

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