Cell

Cell

Volume 50, Issue 4, 14 August 1987, Pages 555-563
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Article
Tumors secreting human TNF/cachectin induce cachexia in mice

https://doi.org/10.1016/0092-8674(87)90028-6Get rights and content

Abstract

Anorexia and weight loss are serious complications that adversely effect the prognosis of cancer patients. It has been suggested that TNF/cachectin may cause cachexia. To determine If TNF/cachectin can induce progressive weight loss in tumor-bearing animals, a clone of the human TNF/cachectin gene was isolated and inserted into a mammalian expression vector. This construct was transfected into CHO cells, and a cell line (CHOTNF-20) that secretes TNF/cachectin was isolated. A cell line (CHOCMV-Neo) that contains the same expression vector without the TNF/cachectin gene was also isolated. Nude mice injected intra-peritoneally with CHOTNF-20 cells died more quickly than mice injected with CHOCMV-Neo cells. Eighty-seven percent of mice inoculated intramuscularly with CHOTNF-20 cells developed severe cachexia and weight loss. All mice bearing CHOCMV-Neo tumors maintained or increased their body weight. We conclude that mice bearing tumors that secrete TNF/cachectin develop progressive wasting and die more quickly than mice bearing control tumors.

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