Clinical Immunology and Immunopathology
Anticardiolipin antibodies in unselected autoimmune rheumatic disease patients
References (34)
- et al.
Lancet
(1984) - et al.
Lancet
(1983) - et al.
Clin. Rheum. Dis.
(1985) - et al.
Amer. J. Med.
(1979) - et al.
Lancet
(1986) - et al.
J. Amer. Med. Assoc.
(1952) - et al.
Clin. Exp. Rheumatol.
(1985) - et al.
Brit. J. Haematol.
(1985) - et al.
Brit. J. Obstet. Gynecol.
(1985) Clin. Exp. Rheumatol.
(1985)
Clin. Exp. Immunol.
Clin. Exp. Immunol.
Arthritis Rheum.
Bull. Rheum. Dis.
Arthritis Rheum.
Arthritis Rheum.
Cited by (110)
Evolution of cardiac dysfunction in patients with antiphospholipid antibodies and/or antiphospholipid syndrome: A 10-year follow-up study
2014, Seminars in Arthritis and RheumatismCitation Excerpt :The following clinical and laboratory parameters were recorded in all patients: ECLAM score for patients with SLE, biopsy-proven kidney involvement, serositis, livedo reticularis, venous and/or arterial thrombosis, pregnancy morbidity [22], systemic hypertension (systolic blood pressure ≥140 mm Hg and/or diastolic pressure ≥90 mm Hg), hyperlipidemia (total cholesterol ≥200 mg/dL and/or triglycerides ≥150 mg/dL), the presence of diabetes mellitus, ANA, complement levels, anti-dsDNA, and antibodies to extractable nuclear antigens. Lupus anticoagulant (detected according to the guidelines of the International Society on Thrombosis and Haemostasis [23]) as well as IgG and IgM isotypes of aCL and anti-β2GPI antibodies (detected on two or more occasions 12 weeks apart) were also recorded and considered positive if their titers were >40 u or >99th percentile of the titers of 100 normal human sera, using solid-phase enzyme immunoassays (ELISAs) [1,24,25]. Medications including immunosuppressives, anticoagulant, and antiplatelet agents, as well anti-arrhythmic and antihypertensive drugs, were recorded.
Comparative study of kidney affection in SLE patients with and without antiphospholipid syndrome
2012, Egyptian RheumatologistCitation Excerpt :Antinuclear and anti-DNA antibodies, C3, and C4 were assessed according to standard methods. Determination of IgG and IgM antibodies to aCL was performed with an enzyme-linked immunosorbent assay [13], Cardiolipin (50 μg/ml) in ethanol (Sigma, St. Louis, MO) was used as antigen on polystyrene micro titer plates (Nunc, Naperville, IL), which were left to dry overnight at 4 °C. After washing with phosphate buffered saline, the nonspecific binding sites were blocked by 10% bovine serum in phosphate buffered saline.
Sjögren's syndrome: Audiological and clinical behaviour in terms of age
2010, Acta Otorrinolaringologica EspanolaAntiphospholipid Antibody Syndrome and Autoimmune Diseases
2008, Hematology/Oncology Clinics of North AmericaCitation Excerpt :Now, with the availability of the phosphodiesterase inhibitor sildenafil and the dual endothelin antagonist bosentan, this is no longer true. Patients who have so-called “idiopathic Raynaud's phenomenon” do not appear to have an increased frequency of aCL [42,43], and CAPS has no unique association with SSC. APS is a vasculopathy, not a vasculitis.
Libman-Sacks Endocarditis in Systemic Lupus Erythematosus: Prevalence, Associations, and Evolution
2007, American Journal of MedicineCitation Excerpt :Anti-DNA antibodies were assayed by enzyme-linked immunosorbent assay, antibodies to extractable nuclear antigens were measured by counter immunoelectrophoresis, and complement concentrations were measured by immunodiffusion.13 Anticardiolipin antibodies (immunoglobulin-G and M) were measured by enzyme-linked immunosorbent assay using cardiolipin (cardiolipin 1649; Sigma, St Louis, Mo; 5 mg/mL in ethanol) as antigen on polystyrene plates, as previously described.13 Lupus anticoagulant was measured by activated thromboplastin time, kaolin clotting time, and dilute Russell’s viper venom time.
- 2
Dr. Gharavi was on leave from the Lupus Research Laboratory, St. Thomas Hospital, Rayne Institute, London, England.
- 3
Dr. Kitridou was on sabbatical leave from the University of Southern California, Los Angeles, CA.