The consensus workshops for the detection of autoantibodies to intracellular antigens in rheumatic diseases

doi:10.1016/0022-1759(91)90369-Q

Journal of Immunological Methods

Volume 140, Issue 2, 1991, Pages 181-189

https://doi.org/10.1016/0022-1759(91)90369-Q Get rights and content

Abstract

In 1988 and in 1989 consensus workshops were organized in order to define the interlaboratory concordance in detecting autoantibody specificities in selected sera from patients with rheumatoid disorders and to determine the possible causes of discrepancies. In total 20 sera were tested for the presence of antibodies against nRNP, Sm, Ro (SS-A), La (SS-B), Scl-70, centromeric antigens, ribosomal RNP and Jo-1. The methods used for detection by the 28 European laboratories who participated included immunofluorescence, counter-immunoelectrophoresis, immunodiffusion, immunoblotting and ELISA.

The results showed that only a combination of two or more techniques was able to detect all specificities with an adequate efficiency. Recommendations to improve the efficiency of autoantibody detection and to standardize laboratory protocols are given.

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The consensus workshops for the detection of autoantibodies to intracellular antigens in rheumatic diseases

Abstract

J. Immunol. Methods

Adv. Immunol.

Dissociation of immune responses to the SS-A (Ro) 52-kD and 60-kD polypeptides in systemic lupus erythematosus and Sjögren's syndrome

Arthritis Rheum.

Definition and clinical relevance of antibodies to nuclear RNP and other nuclear antigens in patients with cryptogenic fibrosing alveolitis

Am. Rev. Respir. Dis.

Identification and chemical synthesis of a ribosomal P protein antigenic determinant in systemic lupus erythematosus

The first international standard for antibodies to double-stranded DNA

Ann. Rheum. Dis.

Characterisation of the SS-B (La) antigen in adenovirus-infected and uninfected HeLa cells

EMBO J.