Immunology of DNA. IV. Quantitative aspects of the Farr assay
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2010, Journal of Immunological MethodsCitation Excerpt :ADA levels were measured in the ABT. Sera were analyzed by isokinetic sucrose gradient centrifugation (Aarden et al., 1976). Sucrose gradients (5–32.9%, w/v, sucrose; Merck) were prepared in PBS, pH 7.4 containing 5 mM EDTA and 0.1% Tween (PET).
A bispecific dsDNA × monoclonal antibody construct for clearance of anti-dsDNA IgG in systemic lupus erythematosus
2001, Journal of Immunological MethodsCitation Excerpt :To determine if the E-AHP TRF assay was generally applicable, we measured the anti-dsDNA IgG levels in 28 SLE patient plasmas (Taylor et al., 1987) and 15 normal plasmas using both the TRF assay and the Farr assay. The Farr assay is accepted as the defining assay for high avidity anti-dsDNA antibodies, since the immune complex must remain intact under the harsh conditions of 50% saturated ammonium sulfate (Aarden et al., 1976a,b; Emlen et al., 1986). The results of the E-AHP TRF assay are shown in Fig. 6A.
Computational models in immunological methods: An historical review
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