Development of an enzyme-linked immunosorbent assay to measure total TIMP-1 (Free TIMP-1 and TIMP-1 in combination with matrix-metalloproteinases) and measurement of TIMP 1 and CRP in serum
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Polydopamine-based molecular imprinted optic microfiber sensor enhanced by template-mediated molecular rearrangement for ultra-sensitive C-reactive protein detection
2020, Chemical Engineering JournalCitation Excerpt :Thus the detection methods for CRP are attracting more and more attention [8,9]. The developed detection methods involve enzyme-linked immunosorbent assay (ELISA) [10], piezoelectric microgravimetry at a quartz crystal microbalance (QCM) [11], field effect transistor (FET) signal transduction [12] and impedance immunoassay [13]. These detection strategies may be restricted by high cost, cumbersome steps, time consuming, etc. [14].
Serological evidence of early remodeling in high-risk non-ST elevation acute coronary syndromes
2008, International Journal of CardiologyCitation Excerpt :Within assay variabilities are < 4% with a < 7% between assay variability. Plasma TIMP-1 was measured in the majority of patients using a modification of the method developed by Plumpton [13], which is a two-step sandwich enzyme linked immunosorbent assay using a commercially available kit — RPN2611 (Amersham Pharmaceuticals, Little Chalfont, UK). Intra and inter assay variabilities for plasma TIMP-1 are 4.0 and 4.4% respectively.
An ELISA for the detection of TIMP-1 based on recombinant single chain Fv fusion proteins
2003, Clinica Chimica ActaRapid monitoring of recombinant protein products: A comparison of current technologies
2002, Trends in Biotechnology