Stimulation of the synthesis of collagenase activator protein in cartilage by a factor present in synovial-conditioned medium

https://doi.org/10.1016/0003-9861(86)90382-6Get rights and content

Abstract

We have purified a low molecular weight protein from medium conditioned by calf synovium with physical and biological properties similar to the leukocyte cytokine interleukin 1 (IL-1). The factor is active in stimulating the synthesis (three- to fivefold) of collagenase activator protein (CAP) by the surface (1–2 mm) of articular cartilage while CAP synthesis in the deeper zones of articular cartilage is not affected. Recombinant mouse IL-1 and commercially available purified human IL-1 are also capable of stimulating cartilage to synthesize and secrete CAP. The synthesis of other proteins, including collagenase, appeared to be unaffected by either the synovial factors or the human and mouse IL-1.

References (22)

  • P.R. Elford et al.

    FEBS Lett

    (1985)
  • M.M. Bradford

    Anal. Biochem

    (1976)
  • B.V. Treadwell et al.

    Arch. Biochem. Biophys

    (1986)
  • C.A. Dinarello

    Rev. Infect. Dis

    (1984)
  • I. Gery et al.

    J. Exp. Med

    (1972)
  • J.K. Heath et al.

    Calcif. Tissue Int

    (1985)
  • P.A. McCroskery et al.

    FEBS Lett

    (1985)
  • K. Deshmukh-Phadke et al.

    Eur. J. Biochem

    (1980)
  • M.B. McGuire-Goldring et al.

    Arthritis Rheum

    (1984)
  • S.C. Ridge et al.

    Arthritis Rheum

    (1980)
  • Cited by (20)

    View all citing articles on Scopus

    This work has been supported in part by NIH Grant AM16265. This is the second of two papers.

    View full text