Summary
We sought to investigate the influence of interleukin-10 (IL10) and IL-6 on the acute phase proteins (APP) in systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). IL-10, IL-6, Creative protein (CRP), alpha-1-acid glycoprotein (AGP), and alpha1 antichymotrypsin (ACT) serum levels were determined in one hundred-eight patients (71 with SLE, 37 with RA).
Quantification of the serum IL-10 level showed increased levels in SLE and RA patients as compared to healthy controls. Serum IL-6 level was found to be elevated in SLE and RA patients. A correlation between IL-10 and IL-6 serum level was found only in SLE. CRP and AGP serum levels were increased in RA as compared to controls, whereas in SLE only AGP was found elevated. A statistically significant correlation between IL-6 serum level and CRP, AGP and ACT was found only in RA. No correlation between IL-10 and serum level of CRP, AGP and ACT was established.
Since IL-10 has a potent immunosuppressive activity, we expected it to be negatively correlated with APP levels. Surprisingly, IL-10 did not correlate with APP either in SLE or RA patients. However, the elevation of IL-10 serum levels in SLE and RA and the correlation between IL-10 and IL-6 in SLE may suggest that IL-10 may play a central role in inflammatory connective tissue diseases.
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References
Fiorentino DF, Bond MW, Mosmann TR. Two types of mouse T helper cells. IV. Th2 clones secrete a factor that inhibits cytokine production by Th1 clones. J Exp Med 1989; 170: 2081–8.
Fiorentino DF, Zlotnik A, Mosmann TR, Howard M, O'Garra A. IL-10 inhibits cytokine production by activated macrophages. J immunol 1991; 147: 3815–22.
Moore KW, O'Garra A, de Waal-Malefyt R, Vieira P, Mosmann TR. Interleukin-10. Annu Rev Immunol 1993; 11: 165–90.
Bogdan C, Vodovotz Y, Nathan C. Macrophage deactivation by interleukin 10. J Exp Med 1991; 174: 1549–55.
Fiorentino DF, Zlotnik A, Vieira P, Mosmann TR, Howard M, Moore KW, O'Garra A. IL-10 acts on the antigen-presenting cell to inhibit cytokine production by Th1 cells. J Immunol 1991; 146: 3444–51.
Howard M, O'Garra A. Biological properties of interleukin-10. Immunol Today 1992; 13: 198–200.
Mallya RK, De Beer FC, Berry H. Correlation of clinical parameters of disease activity in rheumatoid arthritis with serum concentration of CRP and ESR. J Rheumatol 1982; 9: 224–8.
Mackiewicz A, Pawlowski T, Mackiewicz-Pawlowska A, Wiktorowicz K, Mackiewicz S. Microheterogeneity of alpha-1-acid glycoprotein as indicator of rheumatoid arthritis activity. Clin Chim Acta 1987; 163: 185–90.
Gabay C, Roux-Lombard P, de Moerloose P, Dayer JM, Vischer T, Guerne PA. Absence of correlation between interleukin-6 and Creactive protein blood levels in systemic lupus erythematosus compared with rheumatoid arthritis. J Rheumatol 1993; 20: 815–21.
Swaak AJG, van Rooyen A, Aarden LA. IL-6 and acute phase proteins in the disease course of patients with SLE. Rheumatol Int 1989; 8: 263–8.
Hirano T, Akira S, Taga T, Kishimoto T. Biological and clinical aspects of interleukin 6. Immunol Today 1991; 11: 443–9.
Tan EM, Cohen AS, Fries JF, Masi AT, AcShane DJ, Rothfield NF, Schaller JG, Talal N, Winchester RJ. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 1982; 25: 1271–7.
Arnet FC, Edworthy SM, Bloch DA, McShane DJ, Fries JF, Cooper NS, et al. The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum 1988; 31: 315–24.
Klinman MK, Steinberg AD. Inquiry into murine and human lupus. Immunol Rev 1995; 144: 157–93.
Miltenburg AMM, Van Laar JM, De Kniper R, Daha MR, Breedveld FC. T cell clones from human rheumatoid synovial membrane functionally represent the th1 subset. Scand J Immunol 1992; 35: 603–9.
Katsikis PD, Chu CQ, Brennan FM, Maini RN, Feldman M. Immunoregulatory role of interleukin-10 in rheumatoid arthritis. J Exp Med 1994; 179: 1517–27.
Llorente L, Richaud-Patin Y, Wijdenes J. Spontaneous production of IL-10 by B lymphocytes in SLE. Eur Cytokine Netw 1993; 4: 421–30.
Llorente L, Richaud-Patin Y, Fior R. In vivo production of IL-10 by non-T cells in RA, Sjögren syndrome, and SLE. Arthritis Rheum 1994; 37: 1647–55.
Linker-Israeli M, Deans RJ, Wallace DJ, Prehn J, Ozeri-Chen T, Klinenberg JR. Elevated levels of endogenous IL-6 in systemic lupus erythematosus. A putative role in pathogenesis. J Immunol 1991; 147: 117–23.
Sugiyama E, Kuroda A, Hori F, Hori T, Taki H, Arai N, Kobayashi M. Serum interleukin-6 level is a sensitive parameter of disease activity in RA. J Clin Rheumatol 1995; 1: 93–8.
Baumann H, Schendel P. Interleukin-11 regulates the hepatic expression of the same plasma protein gens as interleukin-6. J Biol Chem 1991; 266: 20424–7.
Richards CD, Brown TJ, Shoyab M, Baumann H, Gauldie J. Recombinant oncostatin M stimulates the production of acute phase proteins in HepG2 cells and rat primary hepatocytes in vitro. J Immunol 1992; 148: 1731–6.
Schooltink H, Stoyan T, Roeb E, Heinrich PC, Rose-Jphn S. Ciliary neutrophic factor induces APP expression in hepatocytes. FEBS lett 1992; 314: 280–4.
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Lacki, J.K., Samborski, W. & Mackiewicz, S.H. Interleukin-10 and interleukin-6 in lupus erythematosus and rheumatoid arthritis, correlations with acute phase proteins. Clin Rheumatol 16, 275–278 (1997). https://doi.org/10.1007/BF02238963
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DOI: https://doi.org/10.1007/BF02238963