Abstract
Our laboratory is involved in investigating the role of T-cell receptor (Tcr) in collagen-induced arthritis (CIA). During these studies we found AU/ssJ (H-2 q) mice to be resistant to CIA like SWR (H-2 q), as compared with other H-2 q strains with wild-type Tcr like DBA/1 and B 10. Q. Upon screening with monoclonal antibodies F23.1 and KJ23a, AU/ssJ was found to be F23.1 negative (V β 8 Tcr negative) and KJ23a positive (V β 17a Tcr positive). Southern blot analysis on liver DNA using specific Tcr-V β probes confirmed the deletion of V β 8 gene family and also showed that AU/ssJ mice have deletions of V β 9, V β 13, V β 12, and V β 11 genes of Tcr. Further, these mice show a restriction fragment length polymorphism pattern with V β 10, V β 6, and V β 17 probes similar to SWR mice as compared with 1310 mice. Since SWR and AU/ssJ are from different backgrounds, these studies indicate that specific variable region β chain genes of Tcr are crucial for susceptibility to CIA in mice. Furthermore, these studies identify an additional inbred strain which has also deleted 50% of its Tcr-V β genes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barth, R. K., Kim, B. S., Lan, N. C., Hunkapiller, T., Sobieck, N., Winoto, A., Gershenfield, H., Okada, C., Hansburg, D., Weissman, I., and Hood, L. E.: The murine T cell receptor uses a limited repertoire of expressed V β gene segments. Nature 316: 517–522, 1985
Behlke, M. A., Spinella, D. G., Chou, H. S., Sha, W., Hartl, D. L., and Loh, D. Y.: T cell receptor β-chain expression: dependence on relatively few variable region genes. Science 229: 566–570, 1985
Behlke, M. A., Chou, H. S., Huppi, K., and Loh, D. Y.: Murine T cell receptor mutants with deletions of β-chain variable region genes. Proc Natl Acad Sci USA 83: 767–771, 1986
Behlke, M. A., Henkel, T. J., Anderson, S. J., Lan, N. C., Hood, L. E., Braciale, V. I., Braciale, T. J., and Loh, D. Y.: Expression of a murine polyclonal T cell receptor marker correlate with the use of specific members of the Vβ gene segment subfamily. J Exp Med 165: 257–262, 1987
Banerjee, S., Haqqi, T. M., Luthra, H. S., Stuart, J. M., and David, C. S.: Possible role of Vβ T cell receptor genes in susceptibility to collagen induced arthritis in mice. J Exp Med 167: 832–839, 1988
Cathcart, E. S., Hayes, K. C., Gonnerman, W. A., Lazzari, A. A., and Franzblau, C.: Experimental arthritis in a non-human primate. I. Induction by bovine type II collagen. Lab Invest 54: 26–30, 1986
Chou, H. S., Behlke, M. A., Godambe, S. A., Russell, H., Brooks, C. G., and Loh, D. Y.: T cell receptor genes in an alloreactive CTL clone: implications for rearrangement and germline diversity of variable gene segments. EMBO J 5: 2149–2155, 1986
Chou, H. S., Nelson, C. A., Godambe, S. A., Chaplin, D. D., and Loh, D. Y.: Germline organization of the murine T-cell receptor β-chain genes. Science 238: 545–548, 1987
Feinberg, A. P. and Voglstein, B.: A technique for radiolabelling DNA restrictrion endonuclease fragments to high specific activity. Anal Biochem 137: 266–267, 1984
Festing, M. F. W.: Inbred strains of mice, 10th listing. Mouse Newsletter 79: 20, 1987
Gascoigne, N. R. J., Chien, Y. H., Becker, D. M., Kavaler, J., and Davis, M. M.: Genomic organization and sequence of T cell receptor β-chain constant and joining region genes. Nature 310: 387–391, 1984
Griffiths, M. M., Eichwald, R. J., Martin, J. M., Smith, C. B., and Dewitt, C. W.: Imnmunogenetic control of experimental type II collagen induced arthritis. I. Susceptibility and resistance among inbred strains of rats. Arthritis Rheum 24: 871–879, 1981
Hedrick, S. M., Nielsen, E. A., Kavaler, J., Cohen, D. I., and Davis, M. M.: Sequence relationships between putative T cell receptor polypeptides and immunoglobulins. Nature 308: 153–158, 1984
Kappler, J. W., Roehm, N., Haskins, K., Hannum, C., Herron, L., Kubo, R., Cambier, J., Epstein, R., Cohn, M., Taylor, B., and Marrack, P.: Expression of the antigen specific, major histocompatibility complex restricted receptor on T cells. In H. Cantor, L. Chess, and E. Sercarz (eds.): Regulations of the Immune System, pp. 377–388, A. R. Liss, New York, 1984
Kappler, J. W., Wade, T., White, J., Kushnir, E., Blackman, M., Bill, J., Roehm, N., and Marrack, P.: A T cell receptor V β segment that imparts reactivity to a class II major histocompatibility complex product. Cell 49: 263–271, 1987
Kavaler, J., Davis, M. M., and Chien, Y. H.: Localization of a T cell receptor diversity element. Nature 310: 421–423, 1984
Luthra, H. S., Wooley, P. H., Dillon, A. M., Singh, S. K., Lafuse, W. P., Krco, C. J., Huse, A., Stuart, J. M., Griffiths, M. M., and David, C. S.: Immunogenetics of collagen-induced arthritis (CIA) in mice. A model of autoimmune disease. Ann NY Acad Sci 475: 361, 1986
Malissen, M., Minard, K., Myolsness, S., Kronenberg, M., Goverman, J., Humkapiller, T., Prystowsky, M. B., Hoshikai, Y., Fitch, F., Mak, T. W., and Hood, L. E.: Mouse T-cell antigen receptor: structure and organization of constant and joining gene segments encoding the β-polypeptide. Cell 37: 1101–1110, 1984
Meuer, S., Acuto, D., Hussey, R., Hodgdon, J., Fitzgerald, K., Schlossman, S., and Reinherz, E.: Evidence for the T3 associated 90 kd heterodimer as the T cell antigen receptor. Nature 303: 808–810, 1983
Murray, N. E. and Kaiser, K.: The use of phage lambda replacement vectors in the construction of representative genomic DNA libraries. In D. M. Glover (ed.): DNA Cloning, a Practical Approach, Vol. 1, pp. 1–47, IRL Press, Oxford, Washington, 1985
Reed, K. C. and Mann, D. A.: Rapid transfer of DNA from agarose gel to nylon membrane. Nucleic Acids Res 13: 7201–7221, 1985
Stuart, J. M., Townes, A. S., and Kang, A. H.: Collagen autoimmune arthritis. Annu Rev Immunol 2: 199–218, 1984
Trentham, D. E., Townes, A. S., and Kang, A. H.: Autoinimunity to type II collagen: an experimental model of arthritis. J Exp Med 146: 857–868, 1977
Wooley, P. H., Luthra, H. S., Stuart, J. M., and David, C. S.: Type II collagen induced arthritis in mice. I. Major histocompatibility complex (I-region) linkage and antibody correlates. J Exp Med 154: 688–700, 1981
Wooley, P. H., Dillon, A. M., Luthra, H. S., Stuart, J. M., and David, C. S.: Genetic control of type II collagen-induced arthritis in mice: factors influencing disease susceptibility and evidence for multiple MHC-associated gene control. Transplant Proc 15: 180–185, 1983
Zinkernagel, R. M. and Doherty, P. C.: Restriction of in vitro T cell mediated cytotoxicity in lymphocytic choriomeningitis within a syngeneic or semi-allogenic system. Nature 248: 701–702, 1974
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Haqqi, T.M., Banerjee, S., Jones, W.L. et al. Identification of T-cell receptor V β deletion mutant mouse strain AU/ssJ (H-2 q) which is resistant to collagen-induced arthritis. Immunogenetics 29, 180–185 (1989). https://doi.org/10.1007/BF00373643
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00373643