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Quantitative photometrical assessment of iron deposits in synovial membranes in different joint diseases

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Abstract

We investigated 86 synovial membranes from patients suffering either from rheumatoid arthritis (RA) or osteoarthritis (OA). Iron deposits in the synovial membrane were stained by the Prussian blue reaction, and the amount of stained iron was quantitatively assessed by microscope photometry. We found a statistically significant increase in iron deposits in the synovial membrane of RA patients when compared to OA patients. The amount of iron deposits correlated with the histological subtype of synovitis, those presenting with more exudative and proliferative features showing greater amounts of iron deposits. We also observed an inverse correlation between the haemoglobin concentration and erythrocytes in the serum and the amount of iron in the synovial membrane. From our data we concluded that iron deposits in the synovial membrane can contribute by several mechanisms, including activation of oxygen radicals, to the chronic inflammatory reaction in RA synovitis.

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References

  • Arnett FC, Edworthy SM, Bloch DA, McShane DJ, Fries JF, Cooper NS, Healey LA, Kaplan SR, Liang MH, Luthra HS, Medsger TA, Mitchell DM, Neustadt DH, Pinals RS, Schaller JG, Sharp JT, Wilder RL, Hunder GG (1988) The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum 31:315–325

    Google Scholar 

  • Babior BM, Kipnes RS, Curnutte JT (1973) Biological defense mechanisms. The production by leukocytes of superoxide, a potential bactericidal agent. J Clin Invest 52:741–744

    Google Scholar 

  • Biemond P, Swaak AJG, van Eijk HG, Koster JF (1986) Intraarticular ferritin-bound iron in rheumatoid arthritis. A factor that increases oxygen free radical-induced tissue destruction. Arthritis Rheum 29:1187–1193

    Google Scholar 

  • Blake DR, Gallagher PJ, Potter AR, Bell MJ, Bacon PA (1984) The effect of synovial iron on the progression of rheumatoid disease. A histologic assessment of patients with early rheumatoid synovitis. Arthritis Rheum 27:495–501

    Google Scholar 

  • Cary NR, Sethi D, Brown EA, Erhardt CC, Woodrow DF, Gower PE (1986) Dialysis arthropathy: amyloid or iron. Br Med J Clin Res 293:1392–1394

    Google Scholar 

  • Chambourlier P, De-Laforte C, Bernard PJ, Gabriel B (1977) Intraarticular iron sequestration and rheumatic anemia. Histologic and isotopic study. Sem Hop Paris 53:1053–1058

    Google Scholar 

  • Comporti M (1991) Glutathione depleting agents and lipid peroxidation. Lipids 45:143–169

    Google Scholar 

  • D'Eshougues JR, Delcambre B, Leonardelli J, Defrance D, Rammaert-Guillot M, Rammaert-Guillot B (1971) Secondes vues sur la presencede fer dans la synoviale articularie. Rev Rheum Mal Osteoartic 43:469–473

    Google Scholar 

  • Fassbender HG (1972) Konzept einer Pathosystematik der chronischen Polyarthritis. Z Rheumaforsch 31:129–136

    Google Scholar 

  • Fassbender HG (1975) Pathologie rheumatischer Erkrankungen. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Fassbender HG (1984) Rheumatoide Arthritis. In: Dörr W, Ühlinger E (eds) Spezielle pathologische Anatomie Band 18/I Gelenke und Weichteile. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Feelders RA, Vreugdenhil G, van Dijk JP, Swaak AJ, van Eijk HG (1993) Decreased affinity and number of transferrin receptors on erythroblasts in the anemia of rheumatoid arthritis. Am J Hematol 43:200–204

    Google Scholar 

  • Fritz P, Hoenes J, Rautenstrauch H, Lutz D, Tuczek HV, Mischlinski A, Saal HJ, Klein C, Laschner W (1988) Immunophotometric quantification of extravascular immunoglobulin deposits in the synovial membrane of patients with osteoarthritis and rheumatoid arthritis. Basic Appl Histochem 32:455–469

    Google Scholar 

  • Fritz P, Laschner W, Saal JG, Deichsel G, Tuczek HV, Wegner G (1989) Histological classification of synovitis. Zentralbl Allg Pathol 135:729–741

    Google Scholar 

  • Fritz P, Arold N, Mischlinski A, Wisser H, Oeffinger B, Neuhoff V, Laschner W, König G (1990) Two-dimensional electrophoresis of synovial tissue, synovial fluid and serum in patients with rheumatoid arthritis and related diseases. Rheumatol Int 10: 177–183

    Google Scholar 

  • Fritz P, Klein C, Mischlinski A, Hage C, Dittel KK, Laschner W (1992) Morphometric analysis of the angioarchitecture of the synovial membrane in rheumatoid arthritis and osteoarthritis. Zentralbl Pathol 138:128–135

    Google Scholar 

  • Geiler G, Stiehl P (1974) Zur Bedeutung der Begriffe Basisaktivität und aktuelle Aktivität in der Beurteilung der Synovialmembran bei Rheumatoid-Arthritis. Z Rheumatol 33:73–86

    Google Scholar 

  • Goldie I (1970) Iron deposits in original and regenerated synovial tissue after synovectomy in rheumatoid arthritis. Clin Orthop 68:316–321

    Google Scholar 

  • Griffiths HR, Lunec J (1989) The effect of oxygen free radicals on the carbohydrate moiety of IgG. FEBS Lett 245:95–99

    Google Scholar 

  • Gutteridge JMC, Rowley DA, Hallwill B (1981) Superoxide-dependent formation of hydroxyl radicals in the presence of iron salts: detection of “free” iron in biological systems by using bleomycin-dependent degradation of DNA. Biochem J 199:263–265

    Google Scholar 

  • Hansen TM, Hansen NE, Birgens NE, Holund B, Lorenzen I (1983) Serum ferritin and the assessment of iron deficiency in rheumatoid arthritis. Scand J Rheumatol 12:353–359

    Google Scholar 

  • Hewitt SD, Lunec J, Morris CJ, Blake DR (1987) Effect of free radical altered IgG on allergic inflammation. Ann Rheum Dis 46: 866–874

    Google Scholar 

  • Jasin HH (1987) Cross-linking of immune complexes by human mononuclear phagocytes. Inflammation 11:117–129

    Google Scholar 

  • Kahle P, Saal JG, Schaudt K, Zacher J, Fritz P, Pawlec G (1992) Determination of cytokines in synovial fluids: correlation with diagnosis and histomorphological characteristics of synovial tissue. Ann Rheum Dis 51:731–734

    Google Scholar 

  • Laschner W, Fritz P, Mischlinski A, Mischlinski G, Rautenstrauch H, Saal HJ, Tuczek HV, Wegner G (1988) Klinische Wertigkeit der histomorphologischen Untersuchung von Gelenkkapseln. Z Rheumatol 47:195–200

    Google Scholar 

  • Means RT, Krantz BS (1992) Progress in understanding the pathogenesis of the anemia of chronic disease. Blood 80:1639–1647

    Google Scholar 

  • Morris CJ, Wainwright AC, Steven MM, Blake DR (1984) The nature or iron deposits in haemophilic synovitis. An immunohistochemical, ultrastructural and X-ray microanalytical study. Virchows Arch [B] 404:75–85

    Google Scholar 

  • Morris CJ, Blake DR, Wainwright AC, Steven MM (1986) Relationship between iron deposits and tissue damage in the synovium: an ultrastructural study. Ann Rheum Dis 45:21–26

    Google Scholar 

  • M'Seffar A, Fornasier VL, Fox IH (1977) Arthropathy as the major clinical indicator of occult iron storage disease. JAMA 238: 1825–1828

    Google Scholar 

  • Muirden KD (1970a) The anemia of RA. The significance of iron deposits in the synovial membrane. Aust Ann Med 2:97–104

    Google Scholar 

  • Muirden KD (1970b) Lymph node iron in rheumatoid arthritis. Histology, ultrastructure and chemical concentration. Ann Rheum Dis 29:81–88

    Google Scholar 

  • Ogilvie-Harris DJ, Fornaiser VL (1980) Synovial iron deposition in osteoarthritis and rheumatoid arthritis. J Rheumatol 7:30–36

    Google Scholar 

  • Okada Y, Nakanishi I, Munehiro C, Umeda S, Ichizen H, Masuda S (1984) The presence of siderosomes in synovioblasts (B cells) of chronic spontaneous hemartros. Histologic, electron microscopic, and roentgenographic electron microanalysis studies of three cases. Arch Pathol Lab Med 1208:968–972

    Google Scholar 

  • Palermo C, Maddali-Bongi S, Bianucci G (1986) Relationship between serum ferritin, iron stores and disease activity in rheumatoid arthritis. Ric Clin Lab 16:463–469

    Google Scholar 

  • Parsons JR, Zingler BM, McKeon JJ (1987) Mechanical and histological studies of acute joint hemorrhage. Orthopedics 10: 1019–1026

    Google Scholar 

  • Rajapakse CNA, Holt PJL, Perera BS (1980) Diagnosis of true iron deficiency in RA. Ann Rheum Dis 39:596ff

    Google Scholar 

  • Rao AS, Vigorita VJ (1984) Pigmented villonodular synovitis (giant cell tumor of the tendon sheath and synovial membrane). A review of eighty-one cases. J Bone Joint Surg 66:76–94

    Google Scholar 

  • Richmond J, Gardner DL, Roy LMH (1956) Nature of anemia in rheumatoid arthritis. III. Changes in the bone marrow and their relation to other features of the disease. Ann Rheum Dis 155:217–227

    Google Scholar 

  • Ropes MW, Bennet GA, Corb C, Hacox R, Jessar R (1959) 1958 revision of diagnostic criteria for rheumatoid arthritis. Arthritis Rheum 2:16–19

    Google Scholar 

  • Roux E, Leski M, Vischer TL (1989) Rheumatologic complications in patients treated with intermittent hemodialysis. Schweiz Rundsch Med Prax 78:609–613

    Google Scholar 

  • Schumacher HR, Lotke P, Athreya B, Rothfuss S (1982) Pigmented villonodular synovitis: light and electron microscopic studies. Semin Arthritis Rheum 12:32–43

    Google Scholar 

  • Sethi D, Morgan TC, Brown EA, Cary NR, Erhardt CC, Pazianas M, Maini RN, Woodrow DF, Gower PE (1990) Dialysis arthropathy: a clinical, biochemical, radiological and histological study of 36 patients. Q J Med 77:1061–1082

    Google Scholar 

  • Soren A, Rosenbauer KA, Klein W, Huth F (1973) Morphological examination of the so-called posttraumatic synovitis. Beitr Pathol 150:11–30

    Google Scholar 

  • Willmore LJ, Rubin JJ (1984) Effects on anti-peroxidants in FeCl2-induced lipid peroxidation and focal edema in rat brains. Exp Neurol 83:62–70

    Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, Robert Bosch Hospital, Auerbachstr. 110, D-70376, Stuttgart, Germany

    P. Fritz & C. Wicherek

  2. Department of Haematology, Oncology, Immunology and Rheumatology, Tübingen, Germany

    J. G. Saal

  3. Baumann Klinik, Stuttgart, Germany

    A. König

  4. Department of Orthopaedic Surgery, Karl Olga Krankenhaus, Stuttgart, Germany

    W. Laschner

  5. Kreiskrankenhaus Bad Schussenried, Bad Schussenried, Germany

    H. Rautenstrauch

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  1. P. Fritz
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  2. J. G. Saal
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  3. C. Wicherek
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  4. A. König
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  5. W. Laschner
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  6. H. Rautenstrauch
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Fritz, P., Saal, J.G., Wicherek, C. et al. Quantitative photometrical assessment of iron deposits in synovial membranes in different joint diseases. Rheumatol Int 15, 211–216 (1996). https://doi.org/10.1007/BF00290523

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  • DOI: https://doi.org/10.1007/BF00290523

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