Elsevier

Journal of Autoimmunity

Volume 11, Issue 5, October 1998, Pages 425-429
Journal of Autoimmunity

Regular Article
Serum Anti-β2-glycoprotein I Antibodies from Patients with Antiphospholipid Antibody Syndrome Bind Central Nervous System Cells

https://doi.org/10.1006/jaut.1998.0214Get rights and content

Abstract

Sera from 20 patients with antiphospholipid syndrome (APS), primary or secondary to systemic lupus erythematosus (SLE), or with SLE, were assayed by immunoblot analysis for anti-β2-glycoprotein I antibodies (aβ2-GPI), and by indirect immunofluorescence (IIF) technique for reactivity with astrocyte and neuron cell lines and with histological sections of human brain biopsies and monkey cerebellum. Six sera from healthy donors were studied as a control. Eleven out of the 20 patient sera contained aβ2-GPI and were immunoreactive with astrocytes and neurons, both in culture and in the histological sections, and with the endotheliocytes of the microvessels present in the histological sections. Cell localization and the pattern of immune reaction were similar to those obtained with a monoclonal antibody aβ2-GPI. Eight of the remaining patient sera, found aβ2-GPI, did not react with the nervous substrates (and the control sera), while one exhibited immunoreactivity analogous to the aβ2-GPI+sera. The interference of anticardiolipin antibodies (aCL) in the immunoreactivity with the nervous substrates was excluded since aCL were present in all patient sera and no immune reaction was observed in the histological sections incubated with a monoclonal aCL. Therefore, the binding of aβ2-GPI from patients to cells of the central nervous system (CNS) occurs independently from aCL. This issue may be relevant to further evaluate the potential pathogenetic role of aβ2-GPI in the CNS damage of APS-like conditions.

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Correspondence to: Guido Valesini, Allergologia e Immunologia Clinica III, Policlinico Umberto I, 00161 Roma, Italy. Fax: 39-6-4940594. E-mail:[email protected]

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