Regular ArticleComparisons of Double Immunodiffusion, ELISA, Western Blot and Cape Blot for the Detection of Anti-SSA Antibody: Study of Anti-SSA Prevalence in Systemic Lupus Erythematosus
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Comparison of automated multiplexed bead-based ANA screening assay with ELISA for detecting five common anti-extractable nuclear antigens and anti-dsDNA in systemic rheumatic diseases
2012, Clinica Chimica ActaCitation Excerpt :The positive rates of anti-SSA and anti-SSB in the Sjögren syndrome group of our study (n = 26) were respectively 88.5% (23 cases) and 30.8% (8 cases) using the BioPlex™ 2200 (Table 3), and only 3 discordant results of anti-SSA and anti-SSB were observed between the results by the BioPlex™ 2200 and ELISA. Similar to our results, some previous studies reported that anti-SSA and -SSB were sensitively detected by some ELISA platforms and multiplexed systems when they were performed comparatively [8,9,14,15]. In this study, we evaluated the analytical performance of a new automated multiplex analyzer, the BioPlex™ 2200 system.
Increased sensitivity of autoantibody determination by coupled-particle light-scattering assay by poly(ethylene glycols)-modified beads
2004, Analytica Chimica ActaCitation Excerpt :Sera were considered positive or negative if both assays gave the same results. Discrepancies between the results given by these two analysis methods were resolved by testing the sera by the Western blot method [18]. For the Copalis™ test the cut-off value was calculated by using the average of CTR values of the negative samples and its standard deviation.
Clinical and serological characteristics in 128 patients with systemic lupus erythematosus
2002, Pathologie BiologieNew methods for detection of anti-nuclear antibodies
1998, Clinical Immunology and ImmunopathologySubacute cutaneous lupus erythematosus versus systemic lupus erythematosus: Diagnostic criteria and therapeutic implications
1998, Journal of the American Academy of Dermatology