Regular Article
Regulation of Cellular Adhesion to Extracellular Matrix Proteins by Galectin-3

https://doi.org/10.1006/bbrc.1998.8708Get rights and content

Abstract

The control of cellular adhesion to extracellular matrix proteins is poorly understood. In the present analyses, we set out to test the hypothesis that high galectin-3 concentration on the cell surface downregulates cellular adhesion to the extracellular matrix proteins. Various tumor cell lines were briefly incubated without or with galectin-3 and then allowed to adhere to wells coated with laminin-1, collagen IV and fibronectin. Our data demonstrated that the cells which were incubated with galectin-3 prior to plating had significantly reduced adhesion to extracellular matrix proteins. This inhibition involved the carbohydrate recognition domain of the lectin because adhesion was achieved in the presence of galectin-3 and lactose but not galectin-3 and sucrose. Furthermore we demonstrated that galectin-3 associates with α1β1 integrin in a lactose dependent manner.

References (21)

  • H.J. Woo et al.

    J. Biol. Chem.

    (1990)
  • J. Ochieng et al.

    Biochem. Biophys. Res. Commun.

    (1992)
  • S. Sato et al.

    J. Biol. Chem.

    (1992)
  • F.A. Van den Brule et al.

    Biochem. Biophys. Res. Commun.

    (1995)
  • D.K. Hsu et al.

    J. Biol. Chem.

    (1992)
  • K.Y. Do et al.

    Biochem. Biophys. Res. Commun.

    (1990)
  • S.H. Barondes et al.

    J. Biol. Chem.

    (1994)
  • J. Ochieng et al.

    Biochem. Biophys. Res. Commun.

    (1995)
  • J. Ochieng et al.

    Biochim. Biophys. Acta

    (1998)
  • D.N.W. Cooper et al.

    J. Cell Biol.

    (1990)
There are more references available in the full text version of this article.

Cited by (0)

Abbreviations: CRD, carbohydrate recognition domain; ECM, extracellular matrix; EHS, Englebreth-Holm Swarm; SDS, sodium dodecylsulfate; PAGE, polyacrylamide gel electrophoresis; BSA, bovine serum albumin; PBS, phosphate buffered saline

1

Corresponding author. Fax: (615)327-6119. E-mail:[email protected].

View full text