Regular ArticleHigh-Concentrations of Fibronectin Fragments Cause Short-Term Catabolic Effects in Cartilage Tissue While Lower Concentrations Cause Continuous Anabolic Effects
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2013, Spine JournalType IX collagen interacts with fibronectin providing an important molecular bridge in articular cartilage
2011, Journal of Biological ChemistryCitation Excerpt :These FN fragments induce a catabolic response that results in a severe loss of cartilage proteoglycan, an early characteristic of OA, both in vitro and in vivo (54, 55). However, at much lower concentrations, FN fragments have an anabolic effect on cartilage metabolism, such as promoting an increased release of TGF-β and insulin-like growth factor-1 (56, 57). Therefore, FN and FN fragments are implicated as important regulators of normal cartilage homeostasis and cartilage degeneration during disease progression.
The cartilage chondrolytic mechanism of fibronectin fragments involves MAP kinases: comparison of three fragments and native fibronectin
2008, Osteoarthritis and CartilageCitation Excerpt :We have reported that fibronectin fragments (Fn-fs) have cartilage chondrolytic activities1, elevate matrix metalloproteinase (MMP) expression2–4, suppress proteoglycan (PG) synthesis in chondrocytes and cartilage5–7 and enhance rates of PG loss from cartilage tissue in explant cultures1–3,5–7.
Mixtures of glucosamine and chondroitin sulfate reverse fibronectin fragment mediated damage to cartilage more effectively than either agent alone
2006, Osteoarthritis and CartilageCitation Excerpt :This latter culture system allowed us to measure effects of agents on homeostasis closer to steady state conditions than serum-free and thus, to measure the summation of anabolic and catabolic events. We have shown that in these long-term 21–28 day cultures, that cell viability as measured by DNA content does not decrease by more than 20%, an amount not statistically different than untreated cartilage54. Bovine cartilage was cultured in 10% serum/DMEM with the single agents or with a mixture and with or without 100 nM Fn-f for periods up to 21 or 28 days.
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