IC₅₀ Values for baricitinib and tofacitinib in cytokine-stimulated human PBMC preparations

Cytokine/pSTAT	B cells		CD4+ T cells		CD8+ T cells		NK cells		Monocytes

	Bari	Tofa	Bari	Tofa	Bari	Tofa	Bari	Tofa	Bari	Tofa
	(nM)	(nM)	(nM)	(nM)	(nM)	(nM)	(nM)	(nM)	(nM)	(nM)

IL-2/5	NDS		23	9***	24	9**	43	15***	NDS
IL-4/6	135	61**	50	18***	27	10***	17	6***	50	39*
IL-6/3	NDS		44	41	NDS		NDS		51	42
IL-10/3	82	76	59	48	63	57	71	61	165	121*
IL-15/5	NDS		37	14***	64	24***	73	24***	NDS
IL-21/3	86	28***	63	22***	65	22***	61	21***	NDS
IFN γ/1	17	20	NDS		NDS		NDS		46	54***
IFN α/1	246	195	77	59*	117	91	137	110**	139	99*
IFN α/3	27	19*	25	19*	26	20	25	18	17	12*
IFN α/5	25	18	21	15*	26	18*	NDS		13	9**
G-CSF/3	NDS		NDS		NDS		NDS		69	103**
GM-CSF/5	NDS		NDS		NDS		NDS		116	163**

*p<0.01; **p<0.001; ***p<0.0001. CSF = colony stimulating factor; G = granulocyte; IC₅₀ = half maximum inhibitory concentration; IFN = interferon; IL = interleukin; M = macrophage; NDS = no detectable signal; NK = natural killer; PBMC = peripheral blood mononuclear cells; pSTAT = phosphorylated signal transducers of activatortranscription.