Source of chondrocytes | Cases (n) | Expressing IL18Rα (n) | Expressing IL18Rβ (n) | Responded to IL18 (n) | |
---|---|---|---|---|---|
35 Cycles | 25 + 20 Cycles* | ||||
The numbers in this table are the number of cartilage samples that fit the heading criteria. The expression of IL18Rα and IL18Rβ was determined by RT-PCR. The IL18 responsive chondrocytes were defined as those producing more MMPs after IL18 stimulation. | |||||
*After 25 cycles’ amplification of the cDNA, 2 μl PCR product was amplified for another 20 cycles by using nested primers. | |||||
Normal cartilage | 8 | 4 | 0 | 8 | 2 |
OA cartilage | 14 | 9 | 0 | 14 | 5 |
RA cartilage | 10 | 6 | 0 | 10 | 3 |