Interleukin 6 production (pg/ml) by PBMC of patients with RA and healthy controls at baseline and 24 hours after stimulation with PHA 5 μg/ml. Also given are measurements in density centrifugation isolated PBM of healthy controls 8 hours after stimulation with PHA and PBL 16 hours after stimulation with αCD3/αCD28
| A77-1726 (µmol/l) | PBMC (unstimulated) Baseline | PBMC (stimulated with PHA) 24 Hours | PBM (stimulated with PHA) 8 Hours | PBL (stimulated with αCD3/αCD28) 16 Hours | ||
|---|---|---|---|---|---|---|
| Controls (n = 5) | Patients with RA (n = 3) | Controls (n = 5) | Patients with RA (n = 3) | Controls (n = 2) | Controls (n = 2) | |
| Results are given as mean (SEM). | ||||||
| ND, not done. | ||||||
| 0 | 0 (0) | 110 (11) | 13967 (4937) | 12880 (1640) | 24306 (3761) | 2320 (420) |
| 1 | 0 (0) | 183 (97) | 12033 (3134) | 10700 (680) | ND | ND |
| 10 | 0 (0) | 0 (0) | 15067 (3162) | 10035 (815) | ND | 2070 (185) |
| 25 | 0 (0) | 175 (175) | 14600 (2872) | 11700 (1200) | ND | ND |
| 50 | 0 (0) | 198 (53) | 12633 (4513) | 16745 (4685) | 25325 (2118) | 2110 (340) |
| 100 | 0 (0) | 133 (83) | 21300 (4198) | 16385 (725) | 57203 (18221) | 2370 (255) |