Determination of DNA degradation with PI-Triton staining
| Cells with a subdiploid DNA content (%) | |
|---|---|
| PI staining in a hypotonic buffer. After generation of lymphoblasts, cells were cultured and stained as described in “Material and methods” and fig 1. Each value represents the mean (SEM) of normal healthy donors (n = 6). | |
| IL2 deprivation | |
| 0 h | 8.0 (1.2) |
| 8 h med | 16.0 (1.5) |
| 8 h IL2 | 12.9 (1.5) |
| 8 h+1 h IL2 | 15.8 (1.4) |
| 8 h+16 h IL2 | 15.1 (2.0) |
| 8 h+24 h IL2 | 16.4 (2.0) |
| 24 h med | 21.8 (1.3) |
| 24 h+1 h IL2 | 20.5 (1.3) |
| 24 h+8 h Il-2 | 22.7 (1.9) |
| 24 h IL2 | 12.8 (1.2) |
| Induced apoptosis | |
| 8 h med | 16.0 (1.5) |
| 8 h IL2 | 12.9 (1.5) |
| UV | 68.1 (3.6) |
| UV+IL2 | 64.4 (4.4) |
| Etoposide | 70.3 (3.3) |
| Etoposide+IL2 | 63.9 (2.6) |