Determination of apoptosis with annexin V/propidium iodide staining
| Viable cells (%) | Early apoptotic cells (%) | Late apoptotic cells (%) | Necrotic cells (%) | |
|---|---|---|---|---|
| Annexin V/PI staining of lymphoblasts. After generation of lymphoblasts, cells were cultured and stained with AxV/PI as described in “Material and methods” and fig 1. Each value represents the mean (SEM) of normal healthy donors (n = 6) | ||||
| IL2 deprivation | ||||
| 0 h | 86.5 (3.0) | 2.0 (1.2) | 5.1 (0.9) | 6.4 (1.3) |
| 8 h med | 81.7 (3.5) | 7.8 (1.5) | 6.0 (1.5) | 4.5 (0.6) |
| 8 h IL2 | 83.8 (2.0) | 6.4 (1.4) | 5.4 (1.1) | 4.4 (0.7) |
| 8 h+1 h IL2 | 82.0 (3.0) | 6.6 (1.5) | 6.4 (1.8) | 5.0 (0.7) |
| 8 h+16 h IL2 | 82.8 (3.0) | 3.1 (1.0) | 9.1 (2.6) | 5.0 (1.0) |
| 8 h+24 h IL2 | 81.9 (3.1) | 3.8 (0.6) | 8.9 (1.8) | 5.4 (1.8) |
| 24 h med | 76.9 (3.6) | 6.6 (0.7) | 10.6 (2.9) | 5.9 (1.6) |
| 24 h+1 h IL2 | 77.6 (3.8) | 5.8 (0.9) | 10.8 (2.6) | 5.8 (1.5) |
| 24 h+8 h IL2 | 77.0 (3.8) | 5.3 (0.2) | 11.6 (2.7) | 6.1 (1.5) |
| 24 h IL2 | 84.6 (2.5) | 2.9 (0.9) | 8.1 (2.0) | 4.4 (1.1) |
| Induced apoptosis | ||||
| 8 h med | 81.7 (3.5) | 7.8 (1.5) | 6.0 (1.5) | 4.5 (0.6) |
| 8 h IL2 | 83.8 (2.0) | 6.4 (1.4) | 5.4 (1.1) | 4.4 (0.7) |
| UV | 26.6 (7.5) | 50.6 (5.9) | 12.5 (3.5) | 10.3 (2.3) |
| UV+IL2 | 28.5 (6.7) | 50.8 (4.5) | 10.8 (3.0) | 9.9 (1.8) |
| Etoposide | 24.1 (6.1) | 52.7 (5.1) | 12.1 (2.6) | 11.1 (1.6) |
| Etoposide+IL2 | 29.4 (6.7) | 48.2 (5.6) | 11.5 (2.4) | 10.9 (1.3) |