Effects of p53 gene and immobilisation (IM) on the tibial bone and bone marrow cells
| p53+/+ | p53−/− | |||
|---|---|---|---|---|
| Control | IM | Control | IM | |
| Data are presented as the mean (SD) of five to six mice. | ||||
| *p<0.05 v control p53+/+; †p<0.05 versus immobilised p53+/+ mice, by the Mann-Whitney U test. The values of control p53−/− are not significantly different from those of control p53+/+. | ||||
| BV/TV, bone volume to tissue volume; Tb.N, trabecular number; Tb.Th, trabecular thickness; BFR/BS, ratio of bone formation rate to bone surface; AP positive CFU-f: alkaline phosphatase positive colony formation unit-fibroblast. | ||||
| BV/TV (%) | 12.3 (0.7) | 9.5 (0.9)* | 13.1 (1.2) | 12.4 (0.8)† |
| Tb.N (/mm2) | 4.4 (0.3) | 3.7 (0.5)* | 4.7 (0.5) | 4.5 (0.2)† |
| Tb.Th (µm) | 28.1 (1.7) | 28.5 (1.7) | 30.9 (1.4) | 31.6 (3.5) |
| BFR/BS (µm-%/day) | 39.4 (3.5) | 22.7 (1.9)* | 41.0 (4.1) | 36.3 (3.0)† |
| AP positive CFU-f (No/culture) | 40.2 (4.9) | 30.3 (4.9)* | 46.5 (5.1) | 54.5 (3.0)† |
| Mineralised nodule formation (% of dish area) | 92.5 (4.1) | 71.3 (10.7)* | 90.3 (7.0) | 94.4 (3.9) |