Inhibition of Fas mediated apoptosis of synovial cells by TGFβ1
| Stimuli | Apoptosis | ||||||
|---|---|---|---|---|---|---|---|
| Anti-Fas IgM | TGFβ1 | Hypodiploid DNA (%) | TUNEL (%) | ΔΨm (%) | DEVDase (%) | IETDase (OD) | LEHDase (OD) |
| Rheumatoid synovial cells were cultured with or without TGFβ1 for 48 hours, washed, and further incubated with anti-Fas IgM for 12 hours. After cultivation, the percentage of hypodiploid DNA+ cells, percentage of TUNEL+ cells, ΔΨm, DEVDase activity, IETDase activity, and LEHDase activity were examined as described in the text. Note that apoptotic cell death of synovial cells induced by anti-Fas IgM was significantly suppressed by TGFβ1. | |||||||
| Results are mean (SD) from six individual experiments. | |||||||
| Anti-Fas IgM (−): addition of control mouse IgM. OD of IETDase and LEHDase was described as OD/μg protein. | |||||||
| *p<0.01. | |||||||
| − | − | 1.4 (0.1) | 2.1 (0.1) | 1.0 (0.1) | 1.6 (0.1) | 0.32 (0.03) | 0.34 (0.09) |
| − | + | 1.3 (0.2) | 2.1 (0.2) | 1.0 (0.1) | 1.5 (0.1) | 0.29 (0.03) | 0.31 (0.04) |
| + | − | 60.3 (5.9)* | 45.8 (3.9)* | 61.5 (5.6)* | 62.5 (6.1)* | 0.96 (0.07)* | 0.92 (0.07)* |
| + | + | 14.2 (1.1)* | 12.5 (1.4)* | 14.1 (1.4)* | 12.9 (1.4)* | 0.44 (0.03)* | 0.39 (0.03)* |