Percentage of CD68 positive SFB, skin FB, and gingival FB after intracellular staining of cells fixed with 4.0% PFA and permeabilisation with 0.25% saponin
| mAb | Synovial fibroblasts | Skin fibroblasts | Gingival fibroblasts | |||
|---|---|---|---|---|---|---|
| RA (n = 7) | OA (n = 9) | JT (n = 6) | (n = 12) | (n = 9) | ||
| mAb, monoclonal antibody; RA, rheumatoid arthritis; OA, osteoarthritis; JT, joint trauma. | ||||||
| *p⩽0.05 Mann-Whitney U test versus OA SFB. | ||||||
| †p⩽0.05 Mann-Whitney U test versus RA SFB. | ||||||
| ‡p⩽0.05 Mann-Whitney U test versus JT SFB. | ||||||
| §p⩽0.05 Mann-Whitney U test versus skin FB. | ||||||
| ¶⩽0.05 Mann-Whitney U test versus prolyl 4-hydroxylase. | ||||||
| **p⩽0.05 Mann-Whitney U test versus CD68/KP1. | ||||||
| ††p⩽0.05 Mann-Whitney U test versus CD68/EBM11. | ||||||
| ‡‡p⩽0.05 Mann-Whitney U test versus CD68/PGM1. | ||||||
| Data are expressed as means (SEM). | ||||||
| Prolyl 4-hydroxylase (intracellular) | 3-2B12 | 92.9 (2.0) | 96.8 (1.0) | 99.6 (0.2)*† (n = 5) | 97.6 (0.5)†‡ | 99.7 (0.1)*†§ |
| CD68 (intracellular) | KP1 | 95.1 (1.5) | 98.6 (0.5)† | 99.3 (0.4)† | 97.3 (0.6)‡ | 99.8 (0.0)*†§ |
| CD68 (intracellular) | EBM11 | 93.5 (2.4) (n = 6) | 97.9 (0.6)†(n = 8) | 98.2 (8.4) | 97.0 (0.8) | 99.4 (0.3)*†§** |
| CD68 (intracellular) | PGM1 | 1.2 (0.4)¶**†† | 1.4 (0.2)¶**†† | 0.9 (0.2)¶**†† | 1.1 (0.5)¶**†† | 0.9 (0.1)*¶**†† |
| CD90 (surface) | AS02 | 65.0 (7.5)¶**††‡‡ | 70.8 (8.5)¶**††‡‡ | 93.6 (2.7)*†¶**††‡‡ | 96.3 (1.1)*†**††‡‡ | 99.7 (0.1)*†‡§**††‡‡ |