Precipitation of HEL by SIC at 4°C. Reproduced, with permission, from ref 11. Copyright © 2002 American Society for Microbiology
| Sample No | ||||||
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | |
| Data are the mean (SEM) of three experiments performed in duplicate. | ||||||
| μg SIC offered/50 μg HEL | 200 | 100 | 50 | 25 | 12.5 | 6.3 |
| Molar ratio HEL:SIC | 0.5:1 | 1:1 | 2.2:1 | 4.4:1 | 8.8:1 | 17.5:1 |
| Protein in pellet (μg) | 78.6 (4.8) | 83.9 (6) | 84 (5.7) | 64.6 (4.8) | 35.6 (2.8) | 15.1 (1.3) |
| Total protein precipitated (%) | 31.4 (1.9) | 55.9 (4) | 84 (5.7) | 86.1 (6.4) | 57 (4.5) | 27 (2.3) |