Immunohistochemical scoring | ||||||||
---|---|---|---|---|---|---|---|---|
Treatment | FACS CD19+B220+ cells (% of gated lymphocytes) | ELISA Anti-CII antibodies (OD value) | F4/80+ cells | CD11b+ cells | CD19+ cells | B220+ cells | CD4+ cells | CD8+ cells |
*The lymphocytes were stained with CD19 and B220. Also, supernatant from lymph node cells challenged with CII in vitro was tested in ELISA for CII antibodies. Immunohistochemical scoring (using a scale from 0 (0%) to 8 (88%–100%)) was on sections taken from lymph nodes of mice treated with etoposide or PBS and stained with F4/80, CD11b, CD19, B220, CD4, or CD8. | ||||||||
Etoposide (n=3) | 59.2 (1.2) | 0 (0) | 2 (0) | 2.7 (2.1) | 1.7 (2.1) | 5.3 (0.6) | 3.7 (0.6) | 2 (0) |
PBS (n=3) | 63.7 (4.2) | 1.2 (0.1) | 2.3 (2.3) | 1.7 (1.2) | 5 (1.7) | 6.3 (2.1) | 3 (1.7) | 2.7 (0.6) |