Primer and biotinylated probe sequences for detection of TGFβ1 gene polymorphisms at codon 10 and codon 25
| Primer | Primer sequence | Annealing temperature (°C) |
|---|---|---|
| Sense | 5-CTTCACCAGCTCCATGTCGATAG-3 | 60 |
| Antisense | 5-CTTCACCAGCTCCATGTCGATAG-3 | 60 |
| Codon | Biotinylated TGF β1 probes | Stringency temperatures (°C) |
| 10*C | 5-GCTGCTGCCGCTGCTGC-3 | 58 |
| 10*T | 5-GCTGCTGCTGCTGCTGCT-3 | 58 |
| 25*G | 5-GCCTGGCCGGCCGGCCG-3 | 62 |
| 25*C | 5-GCCTGGCCCGCCGGCCG-3 | 62 |