Effect of anti-inflammatory agents on IL17-induced collagen release from cartilage
| Treatment | No | Inhibition (%) |
|---|---|---|
| Bovine nasal cartilage explants were incubated for 14 days as described in fig 1 with IL17 (50 ng/ml) in the presence of anti-inflammatory agents including IL4 or IL13 at 50 ng/ml; TGFβ1 at 20 ng/ml; IGF-1 at 200 ng/ml. No collagen release occurred when cartilage explants were treated with these anti-inflammatory agents alone. Collagen release, measured as OHPro, was determined as described in the “Materials and methods”. The percentage inhibition values were calculated relative to control- and IL17-induced collagen release such that the collagen release mediated by control and IL17 were designated as 0% and 100%, respectively, for each individual experiment (n = the number of separate cartilage samples that were assessed). For all experiments the Bonferroni's multiple comparison test was used to compare IL17 alone with IL17 + anti-inflammatory agent; the inhibition observed was statistically significant for all treatments (p≤0.01). | ||
| IL17 + IL4 | 4 | 50–90 |
| IL17 + IL13 | 3 | 50–80 |
| IL17 + TGFβ1 | 7 | 75–95 |
| IL17 + IGF1 | 3 | 60–90 |