Effect of increasing the concentration of polymeric sodium metaphosphate (SMP) on human chondrocytes by tetrazolium salt (MTT) assay
| SMP solution2-150(mg/ml) | |||||
|---|---|---|---|---|---|
| 0 | 1 | 2 | 6 | 15 | |
| % Metabolically active cells (mean (SD))2-151 | 100 | 95 (3.2) | 91 (3.6) | 59.4 (4.3) | 50 (7.6) |
↵2-150 SMP was dissolved in phosphate buffered saline (PBS; 280–300 mmol/kg, pH 7.4).
↵2-151 Values were expressed as a percentage of metabolically active cells as compared with controls.
MTT test measures the number of metabolically active cells by a colorimetric technique. The data represent the mean results of four separate experiments. The control cultures were obtained by incubating cells with PBS for 24 hours.